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Literature summary for 1.2.1.8 extracted from

  • Kuaprasert, B.; Silprasit, K.; Horata, N.; Khunrae, P.; Wongpanya, R.; Boonyalai, N.; Vanavichit, A.; Choowongkomon, K.
    Purification, crystallization and preliminary X-ray analysis of recombinant betaine aldehyde dehydrogenase 2 (OsBADH2), a protein involved in jasmine aroma, from Thai fragrant rice (Oryza sativa L.) (2011), Acta Crystallogr. Sect. F, 67, 1221-1223.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Oryza sativa

Crystallization (Commentary)

Crystallization (Comment) Organism
microbatch method, in the presence of NAD+, using 0.1 M HEPES pH 7.4, 24% (w/v) PEG 4000 and 0.2 M ammonium chloride Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-aminobutyraldehyde + NAD+ + H2O Oryza sativa
-
 4-aminobutyric acid + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 Thai fragrant rice
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose affinity column chromatography, and gel filtration Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutyraldehyde + NAD+ + H2O
-
 Oryza sativa 4-aminobutyric acid + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
BADH2
-
 Oryza sativa
betaine aldehyde dehydrogenase 2
-
 Oryza sativa
betaine aldehyde:NAD(P)+ oxidoreductase
-
 Oryza sativa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Oryza sativa

General Information

General Information Comment Organism
metabolism betaine aldehyde dehydrogenase 2 is a key enzyme in the synthesis of fragrance aroma compounds. The extremely low activity of the enzyme in catalyzing the oxidation of acetaldehyde is crucial for the accumulation of the volatile compound 2-acetyl-1-pyrroline in fragrant rice Oryza sativa