Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.85 extracted from

  • Orii, C.; Takenaka, S.; Murakami, S.; Aoki, K.
    Metabolism of 4-amino-3-hydroxybenzoic acid by Bordetella sp. strain 10d: a different modified meta-cleavage pathway for 2-aminophenols (2006), Biosci. Biotechnol. Biochem., 70, 2653-2661.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ complete inhibition at 1 mM Bordetella sp.
Hg2+ complete inhibition at 1 mM Bordetella sp.
iodoacetic acid 69% residual activity at 1 mM Bordetella sp.
N-ethylmaleimide 18% residual activity at 1 mM Bordetella sp.
p-chloromercuribenzoate 41% residual activity at 1 mM Bordetella sp.
Zn2+ 26% residual activity at 1 mM Bordetella sp.

Organism

Organism UniProt Comment Textmining
Bordetella sp.
-
-
-
Bordetella sp. 10d
-
-
-

Purification (Commentary)

Purification (Comment) Organism
streptomycin sulfate precipitation, ammonium sulfate precipitation, DE52 cellulose column chromatography, DEAE-cellulofine column chromatography, and DEAE-Toyopearl column chromatography Bordetella sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:4-amino-3-hydroxybenzoate-grown cell
-
Bordetella sp.
-
culture condition:succinate-glucose-grown cell
-
Bordetella sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
enzyme from cell extract shows a specific activity of 0.0041 units/mg in 100 mM sodium phosphate, pH 7.5, at 24°C. After 17fold purification, the enzyme shows a specific activity of 0.071 units/mg in 100 mM sodium phosphate, pH 7.5, at 24°C, in 100 mM sodium phosphate, pH 7.5, at 24°C Bordetella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O 100% activity Bordetella sp. (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
-
?
acetaldehyde + NAD+ + H2O 113% activity compared to 2-hydroxymuconate-6-semialdehyde Bordetella sp. acetate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O 25% activity compared to 2-hydroxymuconate-6-semialdehyde Bordetella sp. benzoate + NADH + H+
-
?
butyraldehyde + NAD+ + H2O 138% activity compared to 2-hydroxymuconate-6-semialdehyde Bordetella sp. butanoate + NADH + H+
-
?
formaldehyde + NAD+ + H2O 16.5% activity compared to 2-hydroxymuconate-6-semialdehyde Bordetella sp. formate + NADH + H+
-
?
propionaldehyde + NAD+ + H2O 107% activity compared to 2-hydroxymuconate-6-semialdehyde Bordetella sp. propanoate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
NAD+-dependent 2-hydroxy-muconic 6-semialdehyde dehydrogenase
-
Bordetella sp.