KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
putA gene product, different forms by native PAGE with 2 activities: proline oxidase and 1-pyrroline-5-carboxylate dehydrogenase activity | Salmonella enterica subsp. enterica serovar Typhimurium |
132000 | - |
2 * 132000, detergent solubilized, disc gel electrophoresis | Salmonella enterica subsp. enterica serovar Typhimurium |
132000 | - |
1 * 132000, membrane bound in vivo, disc gel electrophoresis | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
putA gene product: enzyme with 2 activities: proline oxidase and 1-pyrroline-5-carboxylate dehydrogenase, which require different reaction conditions | - |
Purification (Comment) | Organism |
---|---|
copurification with proline oxidase | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
104 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
0°C, 70 mM Tris-HCl buffer, pH 8.2, 30% v/v glycerol, several weeks | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NAD+ + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | L-glutamate + NADH | - |
ir | |
additional information | enzyme with 2 activities: proline oxidase and 1-pyrroline-5-carboxylate dehydrogenase, which require different reaction conditions | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 132000, detergent solubilized, disc gel electrophoresis | Salmonella enterica subsp. enterica serovar Typhimurium |
monomer | 1 * 132000, membrane bound in vivo, disc gel electrophoresis | Salmonella enterica subsp. enterica serovar Typhimurium |