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Literature summary for 1.2.1.88 extracted from

  • Strecker, H.J.
    DELTA1-pyrroline-5-carboxylate dehydrogenase (ox liver) (oxidation of DELTA1-pyrroline-5-carboxylate to glutamate) (1971), Methods Enzymol., 17B, 262-265.
No PubMed abstract available
Search for more literature for 1.2.1.88

Inhibitors

Inhibitors Comment Organism Structure
4-aminobutyrate
-
 Bos taurus
5-Aminopentanoate
-
 Bos taurus
6-aminohexanoate
-
 Bos taurus
ADP
-
 Bos taurus
AMP
-
 Bos taurus
arsenite
-
 Bos taurus
ATP
-
 Bos taurus
L-hydroxyproline
-
 Bos taurus
p-hydroxymercuribenzoate
-
 Bos taurus
piperidin-2-one
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
 NAD+
-
 Bos taurus
0.062
-
 1-pyrroline-5-carboxylate with NADP+ Bos taurus
0.12
-
 NADP+
-
 Bos taurus
0.29
-
 1-pyrroline-5-carboxylate with NAD+ Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.55
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
-15°C, 50% loss of activity, 2 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Bos taurus L-glutamate + NADH
-
 ir
1-pyrroline-5-carboxylate + NADP+ + H2O
-
 Bos taurus L-glutamate + NADPH + H+
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 8.6
-
 Bos taurus

Cofactor

Cofactor Comment Organism Structure
NAD+ preferentially Bos taurus
NADP+
-
 Bos taurus