Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-3-phosphoglycerate | competitive | Spinacia oleracea | |
Diamide | - |
Spinacia oleracea | |
iodoacetamide | - |
Spinacia oleracea | |
L-Glyceraldehyde 3-phosphate | - |
Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
NADP+ | - |
Spinacia oleracea | |
0.031 | - |
D-glyceraldehyde 3-phosphate | - |
Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
190000 | 200000 | gel filtration | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde-3-phosphate + NADP+ | Spinacia oleracea | may be part of a system that is responsible for the export of photosynthetically generated NADPH from chloroplast to cytoplasm | D-3-phosphoglycerate + NADPH | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH + H+ | proposed mechanism | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15 | - |
- |
Spinacia oleracea |
Storage Stability | Organism |
---|---|
0-4°C, 50 mM Tris-HCl buffer, pH 7.5, 1 mM EDTA, 10 mM mercaptoethanol, at least 6 months, or precipitated in 60% ammonium sulfate | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde-3-phosphate + NADP+ | absolutely specific for D-isomer | Spinacia oleracea | D-3-phosphoglycerate + NADPH | - |
ir | |
D-glyceraldehyde-3-phosphate + NADP+ | may be part of a system that is responsible for the export of photosynthetically generated NADPH from chloroplast to cytoplasm | Spinacia oleracea | D-3-phosphoglycerate + NADPH | - |
ir |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | absolutely specific for | Spinacia oleracea |