Protein Variants | Comment | Organism |
---|---|---|
E268A | attacking water molecule in the hydrolysis process is poorly activated, can be overcome by the nulceophiles hydrazin and hydroxylamine | Streptococcus mutans |
E268Q | attacking water molecule in the hydrolysis process is poorly activated, can be overcome by the nulceophiles hydrazin and hydroxylamine | Streptococcus mutans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydrazin | protonated and neutral form, compete with the attacking water molecule for the binding site E268, inhibition mechanism | Streptococcus mutans | |
hydroxylamine | protonated and neutral form, compete with the attacking water molecule for the binding site E268, inhibition mechanism | Streptococcus mutans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde-3-phosphate + NADP+ | Streptococcus mutans | - |
D-3-phosphoglycerate + NADPH | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH + H+ | two-step mechanism and kinetics | Streptococcus mutans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde-3-phosphate + NADP+ | - |
Streptococcus mutans | D-3-phosphoglycerate + NADPH | - |
ir | |
D-glyceraldehyde-3-phosphate + NADP+ | two-step mechanism | Streptococcus mutans | D-3-phosphoglycerate + NADPH | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptococcus mutans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Streptococcus mutans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
steady-state rate constant for mutant E268Q and E268A is pH-dependent | Streptococcus mutans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Streptococcus mutans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
hydroxylamine | wild-type enzyme | Streptococcus mutans | |
6.3 | - |
hydrazin | wild-type enzyme | Streptococcus mutans | |
57 | - |
hydroxylamine | mutant E268Q | Streptococcus mutans | |
95 | - |
hydroxylamine | mutant E268A | Streptococcus mutans | |
109 | - |
hydrazin | mutant E268A | Streptococcus mutans | |
254 | - |
hydrazin | mutant E268Q | Streptococcus mutans |