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Literature summary for 1.2.1.9 extracted from

  • Bustos, D.M.; Iglesias, A.A.
    Non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase is post-translationally phosphorylated in heterotrophic cells of wheat (Triticum aestivum) (2002), FEBS Lett., 530, 169-173.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
NADP+ enzyme from leaf before treatment with alkaline phosphatase Triticum aestivum
0.04
-
NADP+ enzyme from endosperm before treatment with alkaline phosphatase Triticum aestivum
0.04
-
NADP+ enzyme from leaf after treatment with alkaline phosphatase Triticum aestivum
0.06
-
NADP+ enzyme from endosperm after treatment with alkaline phosphatase Triticum aestivum
0.101
-
D-Glyceraldehyde-3-phosphate enzyme from leaf before or after treatment with alkaline phosphatase Triticum aestivum
0.118
-
D-Glyceraldehyde-3-phosphate enzyme from endosperm before or after treatment with alkaline phosphatase Triticum aestivum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Triticum aestivum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
240000
-
native enzyme independent of phosphorylation state, gel filtration Triticum aestivum

Organism

Organism UniProt Comment Textmining
Triticum aestivum Q8LK61
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein reversible phosphorylation can regulate NADPH production in the cytosol of heterotrophic plant cells, the phosphorylated enzyme form has similar affinity for substrate but significant lower maximal velocity Triticum aestivum

Purification (Commentary)

Purification (Comment) Organism
-
Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
Triticum aestivum
-
heterotrophic cell
-
Triticum aestivum
-
leaf
-
Triticum aestivum
-
shoot
-
Triticum aestivum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde-3-phosphate + NADP+
-
Triticum aestivum D-3-phosphoglycerate + NADPH
-
?

Synonyms

Synonyms Comment Organism
non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Triticum aestivum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Triticum aestivum