Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | gapN transcription is enhanced by the catabolite control protein A, CcpA | Streptococcus equinus |
Cloned (Comment) | Organism |
---|---|
EcoRI-digested genomic DNA into plasmid pUC18 for sequencing of gapN, into an expression vector for recombinant production of the enzyme in Escherichia coli | Streptococcus equinus |
for expression in Escherichia coli | Streptococcus equinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51100 | - |
- |
Streptococcus equinus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + NADP+ + H2O | Streptococcus equinus | - |
3-phospho-D-glycerate + NADPH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + NADP+ + H2O | Streptococcus equinus | - |
3-phospho-D-glycerate + NADPH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus equinus | Q3C1A6 | - |
- |
Streptococcus equinus | Q3C1A6 | Streptococcus bovis gapN, sequence of gapN gene of S. mutans and S. pyogenes used for PCR primer design, Q59931, Q99Z67 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.001 | - |
growth stage: growth cessation, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
0.008 | - |
growth stage: just before the cessation of growth, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
0.011 | - |
growth stage: later log growth, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
0.014 | - |
strain 12U1-ccpA-, energy substrate glucose, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
0.014 | - |
strain 12U1-ccpA-, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 | Streptococcus equinus |
0.015 | - |
strain 12U1-ccpA-, energy substrate lactose, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
0.015 | - |
strain 12U1-ccpA-, energy substrate lactose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 | Streptococcus equinus |
0.016 | - |
strain 12U1, energy substrate lactose, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
0.016 | - |
strain 12U1, energy substrate lactose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 | Streptococcus equinus |
0.028 | - |
strain 12IU1p, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 | Streptococcus equinus |
0.028 | - |
strain 12U1p, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
0.032 | - |
growth stage: middle log growth, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
0.033 | - |
strain 12U1, energy substrate glucose, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
0.033 | - |
strain 12U1, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 | Streptococcus equinus |
0.039 | - |
recombinant GAPN overexpression strain 12U1gapN-1, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 | Streptococcus equinus |
0.039 | - |
strain 12U1gapN-1, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
0.065 | - |
recombinant GAPN overexpression strain 12U1gapN-2, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 | Streptococcus equinus |
0.065 | - |
strain 12U1gapN-2, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Streptococcus equinus | 3-phospho-D-glycerate + NADPH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Streptococcus equinus | 3-phospho-D-glycerate + NADPH + H+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
GAPN | - |
Streptococcus equinus |
glyceraldehyde-3-phosphate dehydrogenase | - |
Streptococcus equinus |
glyceraldehyde-3-phosphate dehydrogenase (NADP+) | - |
Streptococcus equinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
activity assay at room temperature | Streptococcus equinus |
23 | - |
activity assay at room temperature | Streptococcus equinus |
60 | - |
- |
Streptococcus equinus |
60 | - |
optimal temperature for Streptococcus bovis GAPN | Streptococcus equinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
activity assay | Streptococcus equinus |
8.5 | - |
optimal pH for Streptococcus bovis GAPN | Streptococcus equinus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Streptococcus equinus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptococcus equinus | - |
- |
5.12 |