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Literature summary for 1.2.1.B25 extracted from

  • Domergue, F.; Vishwanath, S.; Joubes, J.; Ono, J.; Lee, J.; Bourdon, M.; Alhattab, R.; Lowe, C.; Pascal, S.; Lessire, R.; Rowland, O.
    Three Arabidopsis fatty acyl-coenzyme a reductases, FAR1, FAR4, and FAR5, generate primary fatty alcohols associated with suberin deposition (2010), Plant Physiol., 153, 1539-1554.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.B25

Cloned(Commentary)

Cloned (Comment) Organism
open reading frame subcloned into yeast expression vector pVT102U Saccharomyces cerevisiae Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q0WRB0
-
-
Arabidopsis thaliana Q39152
-
-
Arabidopsis thaliana Q9LXN3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cauline leaf
-
 Arabidopsis thaliana
-
flower
-
 Arabidopsis thaliana
-
flower slight accumulation Arabidopsis thaliana
-
additional information FAR1 transcript not found in stem and gene expression patterns of the enzyme is associated with known sites of suberin deposition Arabidopsis thaliana
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additional information gene expression patterns of the enzyme is associated with known sites of suberin deposition Arabidopsis thaliana
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root young Arabidopsis thaliana
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root young and mature one Arabidopsis thaliana
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rosette leaf
-
 Arabidopsis thaliana
-
seedling slight accumulation Arabidopsis thaliana
-
silique developing one Arabidopsis thaliana
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silique slight accumulation in developing ones Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a long-chain fatty acyl-CoA + NADPH + H+ recombinant protein nearly exclusively produces C18:0 fatty alcohol Arabidopsis thaliana a long-chain aldehyde + CoA + NADP+ aldehyde intermediate is immediately reduced into the corresponding alcohol, chain lengths of primary alcohols produced by FAR heterologous expression in yeast are consistent with the chain lengths of primary alcohols found in Arabidopsis suberin ?
a long-chain fatty acyl-CoA + NADPH + H+ recombinant protein produces C18:0 and C20:0 fatty alcohols Arabidopsis thaliana a long-chain aldehyde + CoA + NADP+ aldehyde intermediate is immediately reduced into the corresponding alcohol, chain lengths of primary alcohols produced by FAR heterologous expression in yeast are consistent with the chain lengths of primary alcohols found in Arabidopsis suberin ?
a long-chain fatty acyl-CoA + NADPH + H+ recombinant protein produces C18:0 and C22:0 fatty alcohols, C16:0-OH, C20:0-OH, and C24:0-OH also detectable Arabidopsis thaliana a long-chain aldehyde + CoA + NADP+ aldehyde intermediate is immediately reduced into the corresponding alcohol, chain lengths of primary alcohols produced by FAR heterologous expression in yeast are consistent with the chain lengths of primary alcohols found in Arabidopsis suberin ?

Synonyms

Synonyms Comment Organism
FAR1
-
 Arabidopsis thaliana
FAR4
-
 Arabidopsis thaliana
FAR5
-
 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana FAR1 transcript detectable by RT-PCR at 1 h after wounding and peaks at 48 h and remained high after 96 h, further induction after 4 h at salt concentrations of 50, 100, and 200 mM NaCl - FAR1 levels remain induced at 24 h only in the presence of 200 mM NaCl up
Arabidopsis thaliana FAR4 transcript detectable by RT-PCR at 24 h after wounding and peaks at 48 h and remained high after 96 h, further induction after 4 h at salt concentrations of 50, 100, and 200 mM NaCl - induced expression levels remain high after 24 h at all three salt concentrations up
Arabidopsis thaliana FAR5 transcript detectable by RT-PCR at 1 h after wounding and peaks at 48 h and remained high after 96 h, further induction after 4 h at salt concentrations of 50, 100, and 200 mM NaCl - induced expression levels remain high after 24 h at all three salt concentrations up

General Information

General Information Comment Organism
metabolism enzyme is responsible for generating primary fatty alcohols associated with suberin biosynthesis Arabidopsis thaliana