Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | partial inhibition | Methylococcus capsulatus | |
Fe2+ | partial inhibition | Methylococcus capsulatus | |
additional information | the enzyme is insensitive to EDTA, N-ethylmaleimide, cyanide and azide | Methylococcus capsulatus | |
phenylhydrazine | - |
Methylococcus capsulatus | |
Zn2+ | partial inhibition | Methylococcus capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.143 | - |
formaldehyde | 20°C, pH 7.4 | Methylococcus capsulatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | associated | Methylococcus capsulatus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49500 | - |
4 * 49500, SDS-PAGE | Methylococcus capsulatus |
210000 | - |
gel filtration | Methylococcus capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + ferricytochrome b559/569 complex | Methylococcus capsulatus | the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium | formate + ferrocytochrome b559/569 complex | - |
? | |
formaldehyde + ferricytochrome b559/569 complex | Methylococcus capsulatus Bath | the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium | formate + ferrocytochrome b559/569 complex | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
- |
- |
Methylococcus capsulatus Bath | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methylococcus capsulatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:high copper medium-grown cell | - |
Methylococcus capsulatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
146 | - |
- |
Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + dichloroindophenol | formaldehyde is the only substrate that supports enzyme-mediated reduction of dichloroindophenol | Methylococcus capsulatus | formate + reduced dichloroindophenol | - |
? | |
formaldehyde + dichloroindophenol | formaldehyde is the only substrate that supports enzyme-mediated reduction of dichloroindophenol | Methylococcus capsulatus Bath | formate + reduced dichloroindophenol | - |
? | |
formaldehyde + ferricytochrome b559/569 + H2O | - |
Methylococcus capsulatus | formate + ferrocytochrome b559/569 + 2 H+ | - |
r | |
formaldehyde + ferricytochrome b559/569 + H2O | - |
Methylococcus capsulatus Bath | formate + ferrocytochrome b559/569 + 2 H+ | - |
r | |
formaldehyde + ferricytochrome b559/569 complex | - |
Methylococcus capsulatus | formate + ferrocytochrome b559/569 complex | - |
? | |
formaldehyde + ferricytochrome b559/569 complex | the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium | Methylococcus capsulatus | formate + ferrocytochrome b559/569 complex | - |
? | |
formaldehyde + ferricytochrome b559/569 complex | - |
Methylococcus capsulatus Bath | formate + ferrocytochrome b559/569 complex | - |
? | |
formaldehyde + ferricytochrome b559/569 complex | the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium | Methylococcus capsulatus Bath | formate + ferrocytochrome b559/569 complex | - |
? | |
additional information | no activity with: methanol, acetaldehyde, glyoxal, or butyraldehyde | Methylococcus capsulatus | ? | - |
? | |
additional information | no activity with: methanol, acetaldehyde, glyoxal, or butyraldehyde | Methylococcus capsulatus Bath | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 49500, SDS-PAGE | Methylococcus capsulatus |
Synonyms | Comment | Organism |
---|---|---|
DL-FalDH | - |
Methylococcus capsulatus |
formaldehyde-cytochrome b559/569 oxidoreductase | - |
Methylococcus capsulatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
266 | - |
ferricytochrome b559/569 | 20°C, pH 7.4 | Methylococcus capsulatus | |
290 | - |
dichloroindophenol | 20°C, pH 7.4 | Methylococcus capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | pyrroloquinoline quinone-to-subunit stochiometry of approximately 1:1, 3.6 mol of pyrroloquinoline quinone per mol of enzyme | Methylococcus capsulatus |