Cloned (Comment) | Organism |
---|---|
expression of recombinant AtraAOX2 with His6-tag at the C-terminal end in a baculovirus expression system | Amyelois transitella |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-dinitrophenol | 50 microM, 41.7% residual AtraAOX2 activity | Amyelois transitella | |
4-hydroxymercuribenzoate | 50 microM, 55.3% residual AtraAOX2 activity | Amyelois transitella | |
antimycin A | 10 microM, 45.6% residual AtraAOX2 activity | Amyelois transitella | |
beta-ecdysone | 50 microM, 30.1% residual AtraAOX2 activity | Amyelois transitella | |
beta-estradiol | 50 microM, 60.2% residual AtraAOX2 activity | Amyelois transitella | |
Digitonin | 125 microM, 69.9% residual AtraAOX2 activity | Amyelois transitella | |
ethanol | 2% (v/v), 62.1% residual AtraAOX2 activity | Amyelois transitella | |
Isopropanol | 2% (v/v), 67.9% residual AtraAOX2 activity | Amyelois transitella | |
menadione | 100 microM, 63.1% residual AtraAOX2 activity | Amyelois transitella | |
methanol | 2% (v/v), 33.9% residual AtraAOX2 activity | Amyelois transitella | |
additional information | hydroquinone and sodium deoxycholate have no inhibitory effect | Amyelois transitella | |
potassium cyanide | 1 mM, 71.8% residual AtraAOX2 activity | Amyelois transitella | |
Quinacrine | 1 mM, 34.9% residual AtraAOX2 activity | Amyelois transitella | |
rotenone | 10 microM, 89.3% residual AtraAOX2 activity | Amyelois transitella | |
Sodium azide | 5 mM, 52.4% residual AtraAOX2 activity | Amyelois transitella | |
Triton X-100 | 0.01% (v/v), 71.8% residual AtraAOX2 activity | Amyelois transitella |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
SDS-PAGE, AtraAOX2 monomer still shows strong activity | Amyelois transitella |
280000 | - |
Western blot analysis | Amyelois transitella |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amyelois transitella | S5FPI8 | navel orangeworm | - |
Purification (Comment) | Organism |
---|---|
by Ni-NTA column chromatography | Amyelois transitella |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
antenna | male and female | Amyelois transitella | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-hex-2-enal + H2O + O2 | - |
Amyelois transitella | (2E)-hex-2-enoic acid + H2O2 | - |
? | |
(2E)-non-2-enal + H2O + O2 | - |
Amyelois transitella | (2E)-non-2-enoic acid + H2O2 | - |
? | |
acetaldehyde + H2O + O2 | - |
Amyelois transitella | acetic acid + H2O2 | - |
? | |
acrolein + H2O + O2 | results indicate that AtraAOX2 also functions as a xenobiotic-degrading enzyme | Amyelois transitella | ? | - |
? | |
benzaldehyde + H2O + O2 | - |
Amyelois transitella | benzoic acid + H2O2 | - |
? | |
citral + H2O + O2 | low AtraAOX2 activity | Amyelois transitella | (2E)-3,7-dimethylocta-2,6-dienoic acid + H2O2 | - |
? | |
heptanal + H2O + O2 | - |
Amyelois transitella | heptanoic acid + H2O2 | - |
? | |
hexanal + H2O + O2 | - |
Amyelois transitella | hexanoic acid + H2O2 | - |
? | |
additional information | AtraAOX2 shows strong activity on aldehyde substrates derived from plants, but weak activity on Z11Z1316Ald, AtraAOX2 does not degrade the carbamate insecticide aldicarb | Amyelois transitella | ? | - |
? | |
nonanal + H2O + O2 | - |
Amyelois transitella | nonanoic acid + H2O2 | - |
? | |
propanal + H2O + O2 | highest AtraAOX2 activity | Amyelois transitella | propanoic acid + H2O2 | - |
? | |
vanillin + H2O + O2 | low AtraAOX2 activity | Amyelois transitella | vanillic acid + H2O2 | - |
? | |
xanthine + H2O + O2 | lowest AtraAOX2 activity | Amyelois transitella | ? + H2O2 | - |
? | |
Z11Z13-16Ald + H2O + O2 | low AtraAOX2 activity | Amyelois transitella | ? + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 140000, SDS-PAGE | Amyelois transitella |
Synonyms | Comment | Organism |
---|---|---|
antennae-specific aldehyde oxidase | - |
Amyelois transitella |
AtraAOX2 | - |
Amyelois transitella |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | relatively stable from 20 to 60°C but activity drops dramatically at 70°C, AtraAOX2 preheated at 60°C, shows biphasic inactivation, with a rapid decrease from 0 to 10 min, and monotonous decrease thereafter, with an estimated half-life of 33 min | Amyelois transitella |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
enzyme activity tested with three different buffers at pH 8, but no significant difference observed | Amyelois transitella |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | - |
Amyelois transitella |
General Information | Comment | Organism |
---|---|---|
physiological function | AtraAOX2 could be involved in detoxifying plant-derived toxic aldehydes and aldehyde-containing pesticides | Amyelois transitella |