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Literature summary for 1.2.3.4 extracted from

  • Kumar, R.; Hooda, V.; Pundir, C.S.
    Purification and partial characterization of oxalate oxidase from leaves of forage Sorghum (Sorghum vulgare var. KH-105) seedlings (2011), Indian J. Biochem. Biophys., 48, 42-46.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
FAD 55.5% stimulation of activity at 0.5 mM Sorghum bicolor
FAD 55.5% stimulation of activity at 0.5 mM FAD Sorghum bicolor
FMN 45% stimulation of activity at 0.5 mM Sorghum bicolor
NAD+ 16.6% stimulation of activity at 0.5 mM FAD Sorghum bicolor
riboflavin 33.3% stimulation of activity at 0.5 mM Sorghum bicolor

Inhibitors

Inhibitors Comment Organism Structure
diethyldithiocarbamate strong inhibitor, 30.7% residual activity at 0.1 mM Sorghum bicolor
EDTA strong inhibitor, 61.5% residual activity at 0.1 mM Sorghum bicolor
H3PO4 76.9% residual activity at 0.1 mM Sorghum bicolor
additional information not influenced by FeSO4 Sorghum bicolor
Na2SO4 48.4% residual activity at 0.1 mM Sorghum bicolor
NaCN
-
Sorghum bicolor
ZnSO4 61.3% residual activity at 0.1 mM Sorghum bicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
oxalate at pH 4.5 and 37°C Sorghum bicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 76% stimulation of activity at 0.5 mM Cu2+ Sorghum bicolor
additional information the enzyme is unaffected by 0.1 mM NaCl and Ca2+ Sorghum bicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
2 * 62000, SDS-PAGE Sorghum bicolor
124000
-
gel filtration Sorghum bicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxalate + O2 + H+ Sorghum bicolor
-
CO2 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Sorghum bicolor
-
variant KH-105
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Sorghum bicolor

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Sorghum bicolor
-
seedling
-
Sorghum bicolor
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxalate + O2 + H+
-
Sorghum bicolor CO2 + H2O2
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 62000, SDS-PAGE Sorghum bicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Sorghum bicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Sorghum bicolor