Any feedback?
Literature summary for 1.2.4.1 extracted from
- Pyruvate dehydrogenase complex from bovine kidney and heart (1982), Methods Enzymol., 89, 376-386.
General Stability
| General Stability | Organism |
|---|---|
| very sensitive to freezing and thawing | Bos taurus |
| very sensitive to proteolysis, especially during purification | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
pyruvate | similar values | Bos taurus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
MW of native complex isolated from kidney and heart: 7000000 Da and 8500000 Da respectively | Bos taurus |
| 36000 | - |
alpha2,beta2, 2 * 41000 + 2 * 36000 | Bos taurus |
| 41000 | - |
alpha2,beta2, 2 * 41000 + 2 * 36000 | Bos taurus |
| 154000 | - |
- |
Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + lipoamide | Bos taurus | specific for | S-acetyldihydrolipoamide + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4 | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bos taurus | - |
| kidney | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -50°C, 50 mM potassium phosphate buffer, pH 7.0, 1 mM MgCl2, 20 mM thiamine diphosphate, 0.1 mM EDTA, 0.4 mM DTT, 0.1 mM NAD+, several months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + lipoamide | specific for | Bos taurus | S-acetyldihydrolipoamide + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha2,beta2, 2 * 41000 + 2 * 36000 | Bos taurus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
