Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-Dithiopyridine | 1.1 mM, 30% inhibition | Sus scrofa | |
5,5'-dithiobis(2-nitrobenzoate) | 1.1 mM, 35% inhibition | Sus scrofa | |
AgNO3 | 18 mol per mol enzyme, complete inhibition | Sus scrofa | |
Aluminium sulfate | 1.1 mM, 35% inhibition | Sus scrofa | |
Ba2+ | 0.11 mM, 10% inhibition | Sus scrofa | |
Cd2+ | 0.032 mM, complete inhibition | Sus scrofa | |
citrate | noncompetitive inhibition | Sus scrofa | |
Cu2+ | 0.36 mM, complete inhibition | Sus scrofa | |
EDTA | 0.036 mM, 40% inhibition, 0.36 mM, complete inhibition, reversed by addition of excess Mg2+ and Ca2+ | Sus scrofa | |
Mn2+ | 0.72 mM | Sus scrofa | |
N-ethylmaleimide | 1.1 mM, 50% inhibition | Sus scrofa | |
p-chloromercuribenzenesulfonate | 8 mol per mol enzyme, complete inhibition | Sus scrofa | |
p-chloromercuribenzoate | 8 mol per mol enzyme, complete inhibition | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
thiamine diphosphate | in the presence of Ca2+ and Mg2+ | Sus scrofa | |
0.14 | - |
pyruvate | in the presence of Ca2+ and Mg2+ | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Sus scrofa | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can replace Mg2+ | Sus scrofa | |
Ca2+ | 0.36 mM, 5fold activation | Sus scrofa | |
Mg2+ | 0.36 mM, 5fold activation | Sus scrofa | |
Mn2+ | can replace Mg2+ | Sus scrofa | |
Mn2+ | 0.11 mM, 2fold activation | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
calculation from sedimentation and diffusion data, low speed sedimentation equilibrium centrifugation, meniscus depletion method | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + lipoamide | Sus scrofa | in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate | S-acetyldihydrolipoamide + CO2 | - |
? | |
pyruvate + lipoamide | Sus scrofa | low reactivity with 2-ketovalerate | S-acetyldihydrolipoamide + CO2 | - |
? | |
pyruvate + lipoamide | Sus scrofa | low reactivity with 2-ketoisovalerate | S-acetyldihydrolipoamide + CO2 | - |
? | |
pyruvate + lipoamide | Sus scrofa | low reactivity with 2-ketoisocaproate | S-acetyldihydrolipoamide + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
pyruvate dehydrogenase | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-18°C, 50 mM potassium phosphate, pH 7.0, 0.5 mM EDTA, several months, no loss of activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + benzaldehyde | - |
Sus scrofa | (R)-phenylacetylcarbinol | - |
? | |
alpha-ketobutyrate + lipoamide | - |
Sus scrofa | S-propionyldihydrolipoamide + CO2 | - |
? | |
pyruvate + lipoamide | in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate | Sus scrofa | S-acetyldihydrolipoamide + CO2 | - |
? | |
pyruvate + lipoamide | low reactivity with 2-ketovalerate | Sus scrofa | S-acetyldihydrolipoamide + CO2 | - |
? | |
pyruvate + lipoamide | low reactivity with 2-ketoisovalerate | Sus scrofa | S-acetyldihydrolipoamide + CO2 | - |
? | |
pyruvate + lipoamide | low reactivity with 2-ketoisocaproate | Sus scrofa | S-acetyldihydrolipoamide + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
51 | - |
1 min, complete inactivation | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | with Ca2+ as divalent cation | Sus scrofa |
6.5 | 7 | with Mg2+ as divalent cation | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
8.6 | - |
citrate | - |
Sus scrofa |