Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
182000 | 183000 | calculation from sedimentation and diffusion data | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + lipoamide | Escherichia coli | in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate | S-acetyldihydrolipoamide + CO2 | - |
? | |
pyruvate + lipoamide | Escherichia coli | additional reactions of complex, e. g. reduction of K3Fe(CN)6 | S-acetyldihydrolipoamide + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 50 mM phosphate buffer, pH 7.0, several months, no loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-ketobutyrate + Fe(CN)63- + H2O | - |
Escherichia coli | hydroxyacetate + CO2 + Fe(CN)64- | - |
? | |
pyruvate + Fe(CN)63- + H2O | - |
Escherichia coli | CO2 + Fe(CN)64- | - |
? | |
pyruvate + lipoamide | in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate | Escherichia coli | S-acetyldihydrolipoamide + CO2 | - |
? | |
pyruvate + lipoamide | additional reactions of complex, e. g. reduction of K3Fe(CN)6 | Escherichia coli | S-acetyldihydrolipoamide + CO2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Escherichia coli |