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Literature summary for 1.2.4.1 extracted from

  • Reed, L.J.; Willms, C.R.
    Purification and resolution of the pyruvate dehydrogenase complex (Escherichia coli) (1966), Methods Enzymol., 9, 247-265.
No PubMed abstract available

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
182000 183000 calculation from sedimentation and diffusion data Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + lipoamide Escherichia coli in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate S-acetyldihydrolipoamide + CO2
-
?
pyruvate + lipoamide Escherichia coli additional reactions of complex, e. g. reduction of K3Fe(CN)6 S-acetyldihydrolipoamide + CO2
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 50 mM phosphate buffer, pH 7.0, several months, no loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-ketobutyrate + Fe(CN)63- + H2O
-
Escherichia coli hydroxyacetate + CO2 + Fe(CN)64-
-
?
pyruvate + Fe(CN)63- + H2O
-
Escherichia coli CO2 + Fe(CN)64-
-
?
pyruvate + lipoamide in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate Escherichia coli S-acetyldihydrolipoamide + CO2
-
?
pyruvate + lipoamide additional reactions of complex, e. g. reduction of K3Fe(CN)6 Escherichia coli S-acetyldihydrolipoamide + CO2
-
?

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
Escherichia coli