Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.5.1 extracted from

  • Koland, J.G.; Miller, M.J.; Gennis, R.B.
    Reconstitution of the membrane-bound, ubiquinone-dependent pyruvate oxidase respiratory chain of Escherichia coli with the cytochrome d terminal oxidase (1984), Biochemistry, 23, 445-453.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane inner surface of the cytoplasmic membrane and is coupled to the Escherichia coli aerobic respiratory chain Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + ubiquinone-8 Escherichia coli the role of quinones in the pyruvate oxidase system is investigated in this paper acetate + CO2 + ubiquinol-8
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of a minimal respiratory chain consisting of pyruvate oxidase and cytochrome d terminal oxidase plus ubiquinone8 incorporated in phospholipid vesicles. The catalytic velocity of the reconstituted liposome system is about 30% of that observed when the flavoprotein is reconstituted with Escherichia coli membranes Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + ubiquinone-8 the role of quinones in the pyruvate oxidase system is investigated in this paper Escherichia coli acetate + CO2 + ubiquinol-8
-
?

Synonyms

Synonyms Comment Organism
ubiquinione-dependent pyruvate oxidase
-
Escherichia coli

General Information

General Information Comment Organism
physiological function electron transport from pyruvate to oxygen in the liposome system generates a trans-membrane potential of at least 180 mV, negative inside, which is sensitive to the uncouplers carbonyl cyanide p-(trichloromethoxy)phenylhydrazone and valinomycin. A transmembrane potential is also generated by the oxidation of ubiquinol 1 by the terminal oxidase in the absence of the flavoprotein. The pyruvate oxidase can directly reduce ubiquinone 8 within the phospholipid bilayer, menaquinone 8 will not effectively substitute for ubiquinone 8 in this electron-transfer chain, and the cytochrome d terminal oxidase functions as a ubiquinol 8 oxidase and serves as a coupling site in the Escherichia coli aerobic respiratory chain Escherichia coli