Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.5.2 extracted from

  • Ameyama, M.; Osada, K.; Shinagawa, E.; Matsushita, K.; Adachi, O.
    Purification and characterization of aldehyde dehydrogenase of Acetobacter aceti (1981), Agric. Biol. Chem., 45, 1889-1890.
No PubMed abstract available

General Stability

General Stability Organism
instable enzyme, needs a substrate and a detergent as stabilizing agent Acetobacter aceti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
acetaldehyde pH 5.0, 25°C Acetobacter aceti

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Acetobacter aceti 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
1 * 78000, + 1 * 45000 + 1 * 14000, SDS-PAGE Acetobacter aceti
45000
-
1 * 78000, + 1 * 45000 + 1 * 14000, SDS-PAGE Acetobacter aceti
78000
-
1 * 78000, + 1 * 45000 + 1 * 14000, SDS-PAGE Acetobacter aceti

Organism

Organism UniProt Comment Textmining
Acetobacter aceti
-
IFO 3284
-

Purification (Commentary)

Purification (Comment) Organism
-
Acetobacter aceti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + acceptor + H2O potassium ferricyanide, 2,6-dichlorophenolindophenol, phenazine methosulfate, Wurster's blue and nitroblue tetrazolium are utilized as electron acceptors in aldehyde oxidation Acetobacter aceti acetic acid + reduced acceptor
-
?
butanal + acceptor + H2O potassium ferricyanide, 2,6-dichlorophenolindophenol, phenazine methosulfate, Wurster's blue and nitroblue tetrazolium are utilized as electron acceptors in aldehyde oxidation Acetobacter aceti butanoate + reduced acceptor
-
?
additional information no oxidation of formaldehyde Acetobacter aceti ?
-
?
propanal + acceptor + H2O potassium ferricyanide, 2,6-dichlorophenolindophenol, phenazine methosulfate, Wurster's blue and nitroblue tetrazolium are utilized as electron acceptors in aldehyde oxidation Acetobacter aceti propanoate + reduced acceptor
-
?

Subunits

Subunits Comment Organism
trimer 1 * 78000, + 1 * 45000 + 1 * 14000, SDS-PAGE Acetobacter aceti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Acetobacter aceti

Cofactor

Cofactor Comment Organism Structure
cytochrome c the enzyme shows the typical absorption spectrum of c-type cytochrome Acetobacter aceti