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Literature summary for 1.2.5.2 extracted from

  • Zarnt, G.; Schraeder, T.; Andreesen, J.R.
    Degradation of tetrahydrofurfuryl alcohol by Ralstonia eutropha is initiated by an inducible pyrroloquinoline quinone-dependent alcohol dehydrogenase (1997), Appl. Environ. Microbiol., 63, 4891-4898.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.5.2

Activating Compound

Activating Compound Comment Organism Structure
additional information tetrahydrofurfuryl alcohol induces the enzyme, while the ferricyanide-dependent ADH activity is induced during growth on different alcohols except ethanol Cupriavidus necator

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Cupriavidus necator

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for activity, activates Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73600
-
 1 * 73600, SDS-PAGE Cupriavidus necator
79500
-
 gel filtration Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 211fold to homogeneity Cupriavidus necator

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:tetrahydrofurfuryl alcohol-grown cell
-
 Cupriavidus necator
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
39.7
-
 purified enzyme Cupriavidus necator

Storage Stability

Storage Stability Organism
-80°C, stable for prolonged periods in 20 mM Tris-HCl, pH 8.0, containing 5 mM CaCl2 Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + 2 ferricyanide + H2O
-
 Cupriavidus necator acetate + 2 ferrocyanide + 2 H+
-
 ?
butanal + 2 ferricyanide + H2O
-
 Cupriavidus necator butanoate + 2 ferrocyanide + 2 H+
-
 ?
formaldehyde + 2 ferricyanide + H2O
-
 Cupriavidus necator formate + 2 ferrocyanide + 2 H+
-
 ?
furfural + 2 ferricyanide + H2O
-
 Cupriavidus necator 2-furoic acid + 2 ferrocyanide + 2 H+
-
 ?
additional information product determination, overview. The enzyme shows broad substrate specificity converting different primary alcohols, starting from C2 compounds, secondary alcohols, diols, polyethylene glycol 6000, and aldehydes, including formaldehyde, detailed overview Cupriavidus necator ?
-
 ?
pentanal + 2 ferricyanide + H2O
-
 Cupriavidus necator pentanoate + 2 ferrocyanide + 2 H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 73600, SDS-PAGE Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
heme c the quinohemoprotein contains one covalently bound heme c per monomer Cupriavidus necator
pyrroloquinoline quinone the quinohemoprotein contains one pyrroloquinoline quinone per monomer Cupriavidus necator

pI Value

Organism Comment pI Value Maximum pI Value
Cupriavidus necator isoelectric focusing
-
 9.1