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Literature summary for 1.2.5.3 extracted from
- Cloning and molecular characterization of the genes for carbon monoxide dehydrogenase and localization of molybdopterin, flavin adenine dinucleotide, and iron-sulfur centers in the enzyme of Hydrogenophaga pseudoflava (1999), J. Bacteriol., 181, 5581-5590.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes cutM, cutS, and cutL, gene cluster cutMSL, DNA and amino acid sequence determination and anakysis, sequence comparisons, genetic organization | Hydrogenophaga pseudoflava |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Hydrogenophaga pseudoflava |  |
| iron-sulfur center | two [2Fe-2S] clusters in the small subunit | Hydrogenophaga pseudoflava | |
| Molybdenum | - |
Hydrogenophaga pseudoflava | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17752 | - |
x * 87224, large subunit, + x * 30694, medium subunit, + x * 17752, small subunit, sequence calculation | Hydrogenophaga pseudoflava |
| 30694 | - |
x * 87224, large subunit, + x * 30694, medium subunit, + x * 17752, small subunit, sequence calculation | Hydrogenophaga pseudoflava |
| 87224 | - |
x * 87224, large subunit, + x * 30694, medium subunit, + x * 17752, small subunit, sequence calculation | Hydrogenophaga pseudoflava |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hydrogenophaga pseudoflava | P19913 and P19914 and P19915 | large, medium, and small subunits; genes cutM, cutS, and cutL | - |
| Hydrogenophaga pseudoflava DSM 1084 | P19913 and P19914 and P19915 | large, medium, and small subunits; genes cutM, cutS, and cutL | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by ammonium sulfate fractionation, dialysis, and anion exchange chromatography, followed by ultrafiltration, and gel filtration | Hydrogenophaga pseudoflava |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO + 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride + H2O | - |
Hydrogenophaga pseudoflava | CO2 + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride | - |
? | |
| CO + 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride + H2O | - |
Hydrogenophaga pseudoflava DSM 1084 | CO2 + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 87224, large subunit, + x * 30694, medium subunit, + x * 17752, small subunit, sequence calculation | Hydrogenophaga pseudoflava |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Hydrogenophaga pseudoflava |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Hydrogenophaga pseudoflava |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | in the medium subunit | Hydrogenophaga pseudoflava | |
| molybdenum cofactor | in the large subunit | Hydrogenophaga pseudoflava | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Hydrogenophaga pseudoflava | large subunit, sequence calculation | - |
5.88 |
| Hydrogenophaga pseudoflava | small subunit, sequence calculation | - |
7.77 |
| Hydrogenophaga pseudoflava | medium subunit, sequence calculation | - |
8.43 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the sequences of CutMSL are highly conserved in CO-DHs and other molybdenum-containing hydroxylases | Hydrogenophaga pseudoflava |
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