Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.7.1 extracted from

  • Ikeda, T.; Ochiai, T.; Morita, S.; Nishiyama, A.; Yamada, E.; Arai, H.; Ishii, M.; Igarashi, Y.
    Anabolic five subunit-type pyruvate:ferredoxin oxidoreductase from Hydrogenobacter thermophilus TK-6 (2006), Biochem. Biophys. Res. Commun., 340, 76-82.
    View publication on PubMed
Search for more literature for 1.2.7.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Hydrogenobacter thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
8900
-
 2 * 21300, subunit PorG, 2 * 44300, subunit PorA, 2 * 32300, subunit PoB, 2 * 26100, subunit PorG, 2 * 8900, subunit PorE, calculated and SDS-PAGE Hydrogenobacter thermophilus
21300
-
 2 * 21300, subunit PorG, 2 * 44300, subunit PorA, 2 * 32300, subunit PoB, 2 * 26100, subunit PorG, 2 * 8900, subunit PorE, calculated and SDS-PAGE Hydrogenobacter thermophilus
26100
-
 2 * 21300, subunit PorG, 2 * 44300, subunit PorA, 2 * 32300, subunit PoB, 2 * 26100, subunit PorG, 2 * 8900, subunit PorE, calculated and SDS-PAGE Hydrogenobacter thermophilus
32300
-
 2 * 21300, subunit PorG, 2 * 44300, subunit PorA, 2 * 32300, subunit PoB, 2 * 26100, subunit PorG, 2 * 8900, subunit PorE, calculated and SDS-PAGE Hydrogenobacter thermophilus
44300
-
 2 * 21300, subunit PorG, 2 * 44300, subunit PorA, 2 * 32300, subunit PoB, 2 * 26100, subunit PorG, 2 * 8900, subunit PorE, calculated and SDS-PAGE Hydrogenobacter thermophilus
265000
-
 gel filtration Hydrogenobacter thermophilus

Organism

Organism UniProt Comment Textmining
Hydrogenobacter thermophilus D3DJK0 and D3DJJ8 D3DJK0 i.e. alpha-subunit PorA, D3DJJ8 i.e. gamma-subunit PorG
-
Hydrogenobacter thermophilus TK-6 / IAM 12695 D3DJK0 and D3DJJ8 D3DJK0 i.e. alpha-subunit PorA, D3DJJ8 i.e. gamma-subunit PorG
-

Subunits

Subunits Comment Organism
decamer 2 * 21300, subunit PorG, 2 * 44300, subunit PorA, 2 * 32300, subunit PoB, 2 * 26100, subunit PorG, 2 * 8900, subunit PorE, calculated and SDS-PAGE Hydrogenobacter thermophilus
More subunits PorA, PorB, PorG, and PorE are similar to the alpha, beta, gamma, and delta subunits of the four subunit-type 2-oxoacid oxidoreductases, respectively, and have conserved specific motifs. PorD has no specific motifs but is essential for the expression of the active enzyme. For recombinant enzyme, maximal activity is obtained when all five of the genes, porEDABG, are expressed. In the absence of porD, no active enzyme is produced Hydrogenobacter thermophilus

Synonyms

Synonyms Comment Organism
PorEDABG five-subunit type enzyme Hydrogenobacter thermophilus