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Literature summary for 1.2.7.1 extracted from

  • Menon, A.L.; Hendrix, H.; Hutchins, A.; Verhagen, M.F.; Adams, M.W.
    The delta-subunit of pyruvate ferredoxin oxidoreductase from Pyrococcus furiosus is a redox-active, iron-sulfur protein: evidence for an ancestral relationship with 8Fe-type ferredoxins (1998), Biochemistry, 37, 12838-12846.
    View publication on PubMed
Search for more literature for 1.2.7.1

Cloned(Commentary)

Cloned (Comment) Organism
the gene encoding the delta-subunit is expressed in Escherichia coli Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme contains at least two [4Fe-4S] clusters. The purified and reconstituted delta subunit contains 8 Fe mol/mol Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + CoA + 2 oxidized ferredoxin Pyrococcus furiosus the enzyme catalyzes the final oxidative step in carbohydrate fermentation in which pyruvate is oxidized to acetyl-CoA and CO2, coupled to the reduction of ferredoxin acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
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 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinantly expressed delta subunit is purified after reconstitution with iron and sulfide. The reconstituted delta-subunit is monomeric with a mass of 11879 Da as determined by mass spectrometry. The purified and reconstituted delta subunit contains 8 Fe mol/mol and remains intact when incubated at 85 °C for 2 h Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + CoA + 2 oxidized ferredoxin the enzyme catalyzes the final oxidative step in carbohydrate fermentation in which pyruvate is oxidized to acetyl-CoA and CO2, coupled to the reduction of ferredoxin Pyrococcus furiosus acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?

Subunits

Subunits Comment Organism
More the enzyme is composed of two catalytic units of molecular mass 120000 Da. Each unit consists of four subunits, alpha/beta/gamma/delta, with masses of approximately 44000 Da, 36000 Da, 20000 Da, and 12000 Da, respectively, and contains at least two [4Fe-4S] clusters. The recombinantly expressed and reconstituted delta subunit is monomeric with a mass of 11879 Da Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
POR
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 Pyrococcus furiosus
pyruvate ferredoxin oxidoreductase
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 Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 the recombinantly expressed, purified and reconstituted delta subunit is stable for 2 h Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center the enzyme contains at least two [4Fe-4S] clusters. The purified and reconstituted delta subunit contains 8 Fe mol/mol Pyrococcus furiosus