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Literature summary for 1.2.7.1 extracted from
- Characterization of an ancestral type of pyruvate ferredoxin oxidoreductase from the hyperthermophilic bacterium, Thermotoga maritima (1994), Biochemistry, 33, 1000-1007.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | stimulatory | Thermotoga maritima |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory: carbon monoxide, sodium cyanide, sodium fluoride, sodium azide | Thermotoga maritima | |
| Sodium nitrite | - |
Thermotoga maritima | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.34 | - |
CoA | pH 8.2, 80°C | Thermotoga maritima | |
| 14.5 | - |
pyruvate | pH 8.2, 80°C | Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13000 | - |
x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE | Thermotoga maritima |
| 23000 | - |
x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE | Thermotoga maritima |
| 34000 | - |
x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE | Thermotoga maritima |
| 43000 | - |
x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE | Thermotoga maritima |
| 155000 | - |
gel fitlration | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| 25°C, half-life in presence of air 70 min | Thermotoga maritima |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification under strictly unaerobic conditions | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | CoA is absolutely required. No substrates: 2-oxoglutarate, phenyl pyruvate, or indolyl pyruvate | Thermotoga maritima | ? | - |
? | |
| pyruvate + CoA + oxidized methyl viologen | - |
Thermotoga maritima | acetyl-CoA + CO2 + reduced methyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life 15 h | Thermotoga maritima |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| [4Fe-4S]-center | proteins contains at least two ferredoxin-type [4Fe-4S] clusters per molecule. Km value for ferredoxin is 0.026 microM | Thermotoga maritima | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 54 | - |
Sodium nitrite | pH 8.2, 80°C | Thermotoga maritima | |
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