KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
CoA | pH 8.4, 80°C | Pseudothermotoga hypogea | |
0.063 | - |
CoA | pH 8.4, 80°C | Thermotoga maritima | |
0.13 | - |
pyruvate | pH 8.4, 80°C | Pseudothermotoga hypogea | |
0.14 | - |
oxidized methyl viologen | pH 8.4, 80°C | Pseudothermotoga hypogea | |
0.4 | - |
pyruvate | pH 8.4, 80°C | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13000 | - |
1 * 46000 + 1 * 35000 + 1 * 23000 + 1 * 13000, SDS-PAGE | Thermotoga maritima |
14000 | - |
1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE | Pseudothermotoga hypogea |
22000 | - |
1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE | Pseudothermotoga hypogea |
23000 | - |
1 * 46000 + 1 * 35000 + 1 * 23000 + 1 * 13000, SDS-PAGE | Thermotoga maritima |
35000 | - |
1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE | Pseudothermotoga hypogea |
35000 | - |
1 * 46000 + 1 * 35000 + 1 * 23000 + 1 * 13000, SDS-PAGE | Thermotoga maritima |
45000 | - |
1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE | Pseudothermotoga hypogea |
46000 | - |
1 * 46000 + 1 * 35000 + 1 * 23000 + 1 * 13000, SDS-PAGE | Thermotoga maritima |
156000 | - |
gel filtration | Thermotoga maritima |
157000 | - |
gel filtration | Pseudothermotoga hypogea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudothermotoga hypogea | V9NGI4 and V9NFX1 and V9NGQ2 and V9NHA1 | V9NGI4, i.e. subunit PorB, V9NFX1, i.e. subunit PorD, V9NGQ2 i.e. subunit PorG, V9NHA1 i.e. subunit PorA, bifunctional pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase | - |
Thermotoga maritima | Q56317 and 05650 and Q56316 and O05651 | Q56317, i.e. subunit PorB, O05650, i.e. subunit PorC, Q56316 i.e. subunit PorD, O05651 i.e. subunit PorA, bifunctional pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase | - |
Thermotoga maritima DSM 3109 | Q56317 and 05650 and Q56316 and O05651 | Q56317, i.e. subunit PorB, O05650, i.e. subunit PorC, Q56316 i.e. subunit PorD, O05651 i.e. subunit PorA, bifunctional pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase | - |
Oxidation Stability | Organism |
---|---|
both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity are extremely oxygen sensitive, 50% loss of activity upon exposure to air at room temperature within 15 min | Thermotoga maritima |
both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity are extremely oxygen sensitive, 50% loss of activity upon exposure to air at room temperature within 15 min | Pseudothermotoga hypogea |
Purification (Comment) | Organism |
---|---|
purified enzyme shos a brownish colour characteristic of iron-sulfur containing proteins | Thermotoga maritima |
purified enzyme shows a brownish colour characteristic of iron-sulfur containing proteins | Pseudothermotoga hypogea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
78 | - |
pH 8.4, 80°C | Thermotoga maritima |
119 | - |
pH 8.4, 80°C | Pseudothermotoga hypogea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA + 2 oxidized methyl viologen | - |
Thermotoga maritima | acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+ | - |
? | |
pyruvate + CoA + 2 oxidized methyl viologen | - |
Pseudothermotoga hypogea | acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+ | - |
? | |
pyruvate + CoA + 2 oxidized methyl viologen | - |
Thermotoga maritima DSM 3109 | acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE | Pseudothermotoga hypogea |
tetramer | 1 * 46000 + 1 * 35000 + 1 * 23000 + 1 * 13000, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
pyruvate decarboxylase activity | Pseudothermotoga hypogea |
90 | - |
- |
Thermotoga maritima |
90 | - |
pyruvate ferredoxin oxidoreductase activity | Pseudothermotoga hypogea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life of pyruvate decarboxylase activity 2 h, of pyruvate ferredoxin oxidoreductase activity 3 h | Pseudothermotoga hypogea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity | Thermotoga maritima |
8.4 | - |
both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity | Pseudothermotoga hypogea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | pyruvate ferredoxin oxidoreductase activity is strictly CoA-dependent, desulfo-CoA does not serve as cofactor | Thermotoga maritima | |
CoA | pyruvate ferredoxin oxidoreductase activity is strictly CoA-dependent, desulfo-CoA does not serve as cofactor. The pyruvate decarboxylase activity accepts desulfo-CoA | Pseudothermotoga hypogea |