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Literature summary for 1.2.7.11 extracted from

  • Fukuda, E.; Wakagi, T.
    Substrate recognition by 2-oxoacid:ferredoxin oxidoreductase from Sulfolobus sp. strain 7 (2002), Biochim. Biophys. Acta, 1597, 74-80.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic parameters of mutant enzymes Sulfolobus sp.
0.28
-
pyruvate pH 7.5, 80°C, natural and recombinant wild-type enzyme Sulfolobus sp.
0.45
-
2-oxobutyrate pH 7.5, 80°C, recombinant wild-type enzyme Sulfolobus sp.
0.48
-
2-oxobutyrate pH 7.5, 80°C, natural enzyme Sulfolobus sp.
0.85
-
2-oxoglutarate pH 7.5, 80°C, recombinant wild-type enzyme Sulfolobus sp.
0.87
-
2-oxoglutarate pH 7.5, 80°C, natural enzyme Sulfolobus sp.

Organism

Organism UniProt Comment Textmining
Sulfolobus sp.
-
-
-
Sulfolobus sp. 7
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutyrate + CoA + oxidized ferredoxin kcat/Km for 2-oxobutyrate is 60% compared to the kcat/Km-value for pyruvate, recombinant wild-type enzyme Sulfolobus sp. propanoyl-CoA + CO2 + reduced ferredoxin
-
?
2-oxobutyrate + CoA + oxidized ferredoxin kcat/Km for 2-oxobutyrate is 60% compared to the kcat/Km-value for pyruvate, recombinant wild-type enzyme Sulfolobus sp. 7 propanoyl-CoA + CO2 + reduced ferredoxin
-
?
2-oxoglutarate + CoA + 2 oxidized ferredoxin kcat/Km for 2-oxoglutarate is 12% compared to the kcat/Km-value for pyruvate, recombinant wild-type enzyme Sulfolobus sp. succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
?
2-oxoglutarate + CoA + 2 oxidized ferredoxin kcat/Km for 2-oxoglutarate is 12% compared to the kcat/Km-value for pyruvate, recombinant wild-type enzyme Sulfolobus sp. 7 succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
?
pyruvate + CoA + 2 oxidized ferredoxin
-
Sulfolobus sp. acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
?
pyruvate + CoA + 2 oxidized ferredoxin
-
Sulfolobus sp. 7 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
assay at Sulfolobus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Sulfolobus sp.