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Literature summary for 1.2.7.11 extracted from

  • Luo, J.; Fukuda, E.; Takase, H.; Fushinobu, S.; Shoun, H.; Wakagi, T.
    Identification of the lysine residue responsible for coenzyme A binding in the heterodimeric 2-oxoacid:ferredoxin oxidoreductase from Sulfolobus tokodaii, a thermoacidophilic archaeon, using 4-fluoro-7-nitrobenzofurazan as an affinity label (2009), Biochim. Biophys. Acta, 1794, 335-340.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant enzymes K125A and K173A in Escherichia coli Sulfurisphaera tokodaii

Protein Variants

Protein Variants Comment Organism
K125A the mutant enzyme shows a large increase in the Km-value for CoA and shows poor inactivation by 4-fluoro-7-nitrobenzofurazan, compared with K173A and wild type enzyme Sulfurisphaera tokodaii
K173A the mutant enzyme shows a large increase in the Km-value for CoA and shows poor inactivation by 4-fluoro-7-nitrobenzofurazan, compared with K173A and wild type enzyme Sulfurisphaera tokodaii

Inhibitors

Inhibitors Comment Organism Structure
4-fluoro-7-nitrobenzofurazan 0.001 mM inhibitor with 0.01 mM enzyme decreases the activity by about 20% and 0.003 mM inhibitor with 0.01 mM enzyme decreases the activity by around 50%. Inactivation is prevented by CoA Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
CoA pH 8.5, 80°C, mutant enzyme K173A Sulfurisphaera tokodaii
0.074
-
CoA pH 8.5, 80°C, wild-type enzyme Sulfurisphaera tokodaii
1.17
-
CoA pH 8.5, 80°C, mutant enzyme K125A Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + CoA + 2 oxidized ferredoxin Lys125 in subunit b is the critical residue that interacts with CoA Sulfurisphaera tokodaii acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
?

Synonyms

Synonyms Comment Organism
StOFOR
-
Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
assay at Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Sulfurisphaera tokodaii