Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-bipyridyl | competitive to methyl viologen | Thermococcus sp. | |
acetaldehyde | 50% inhibition at 5 mM | Thermococcus sp. | |
aldehydes | substrate inhibition at high concentrations, overview | Thermococcus sp. | |
arsenite | 50% inhibition at 0.5 mM | Thermococcus sp. | |
crotonaldehyde | 50% inhibition at 20 mM | Thermococcus sp. | |
iodoacetate | 50% inhibition at 0.5 mM | Thermococcus sp. | |
isovalerylaldehyde | 50% inhibition at 4 mM | Thermococcus sp. | |
p-chloromercuribenzoate | - |
Thermococcus sp. | |
phenylacetaldehyde | 50% inhibition at 7 mM | Thermococcus sp. | |
propionaldehyde | 50% inhibition at 0.6 mM | Thermococcus sp. | |
Zn2+ | 50% inhibition at 0.25 mM | Thermococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
oxidized ferredoxin | ES-1 ferredoxin | Thermococcus sp. | |
0.01 | - |
oxidized ferredoxin | pH 8.4, 85°C, with crotonaldehyde | Thermococcus sp. | |
0.016 | - |
acetaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.028 | - |
isovalerylaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.05 | - |
Indoleacetaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.057 | - |
benzaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.065 | - |
Salicylaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.076 | - |
phenylacetaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.13 | - |
crotonaldehyde | pH 8.4, 45°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.14 | - |
crotonaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.15 | - |
propionaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
0.17 | - |
benzyl viologen | pH 8.4, 85°C, with crotonaldehyde | Thermococcus sp. | |
0.2 | - |
glyceraldehyde | pH 8.4, 45°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
1.42 | - |
formaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
1.8 | - |
acetate | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
2.9 | - |
methyl viologen | pH 8.4, 85°C, with crotonaldehyde | Thermococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.7 gatoms per subunit | Thermococcus sp. | |
Fe2+ | contains 1 iron-sulfur cluster [4Fe-4S] per subunit | Thermococcus sp. | |
Mg2+ | contains 1 Mg-atom per subunit | Thermococcus sp. | |
Molybdenum | - |
Thermococcus sp. | |
Tungsten | 1 tungsten per subunit | Thermococcus sp. | |
Zn2+ | 0.2 gatoms per subunit | Thermococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
2 * 67000, SDS-PAGE | Thermococcus sp. |
105000 | - |
gel filtration | Thermococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an aldehyde + H2O + oxidized ferredoxin | Thermococcus sp. | enzyme oxidizes aldehydes generated during amino acid catabolism | an acid + H+ + reduced ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus sp. | - |
hyperthermophilic archaeon | - |
Thermococcus sp. ES1 | - |
hyperthermophilic archaeon | - |
Oxidation Stability | Organism |
---|---|
very sensitive to O2, after 1 h at 60°C 3% activity remains, after 1 h at 4°C 39% activity remains | Thermococcus sp. |
Purification (Comment) | Organism |
---|---|
about 120fold, to homogeneity | Thermococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
238 | - |
purified enzyme | Thermococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | acetate + H+ + reduced benzyl viologen | - |
ir | |
acetaldehyde + H2O + oxidized ferredoxin | best substrate | Thermococcus sp. | acetate + H+ + reduced ferredoxin | - |
ir | |
acetate + H+ + reduced methyl viologen | very low activity | Thermococcus sp. | acetaldehyde + H2O + oxidized methyl viologen | - |
ir | |
acetate + H+ + reduced methyl viologen | below pH 6.0 | Thermococcus sp. | acetaldehyde + H2O + oxidized methyl viologen | - |
ir | |
an aldehyde + H2O + oxidized ferredoxin | - |
Thermococcus sp. | an acid + H+ + reduced ferredoxin | - |
ir | |
an aldehyde + H2O + oxidized ferredoxin | enzyme oxidizes aldehydes generated during amino acid catabolism | Thermococcus sp. | an acid + H+ + reduced ferredoxin | - |
? | |
benzaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | benzoic acid + H+ + reduced benzyl viologen | - |
ir | |
crotonaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | crotonate + H+ + reduced benzyl viologen | - |
ir | |
crotonaldehyde + H2O + oxidized ferredoxin | - |
Thermococcus sp. | crotonate + H+ + reduced ferredoxin | - |
r | |
formaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | formate + H+ + reduced benzyl viologen | - |
ir | |
glyceraldehyde + H2O + oxidized methyl viologen | very poor substrate | Thermococcus sp. | glycerate + H+ + reduced methyl viologen | - |
? | |
indoleacetaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | indoleacetate + H+ + reduced benzyl viologen | - |
ir | |
isovalerylaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | isovalerate + H+ + reduced benzyl viologen | - |
ir | |
additional information | acid reduction is strongly dependent on the overall reduction potential, therfore reverse reaction is only possible with reduced methyl viologen at a low rate | Thermococcus sp. | ? | - |
? | |
phenylacetaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | phenylacetate + H+ + reduced benzyl viologen | - |
ir | |
propionaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | propionate + H+ + reduced benzyl viologen | - |
ir | |
salicylaldehyde + H2O + oxidized benzyl viologen | - |
Thermococcus sp. | salicylic acid + H+ + reduced benzyl viologen | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 67000, SDS-PAGE | Thermococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
assay at | Thermococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is thermostable | Thermococcus sp. |
23 | - |
pure enzyme, 2 mg/ml, 50 mM Tris-HCl, pH 8.0, 2 mM sodium dithionite, 2 mM DTT, 10% v/v glycerol, exposure to air, irreversible loss of 50% activity after 1 min | Thermococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
acetate | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
2.8 | - |
glyceraldehyde | pH 8.4, 45°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
13 | - |
Salicylaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
22 | - |
crotonaldehyde | pH 8.4, 45°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
55 | - |
Indoleacetaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
62 | - |
methyl viologen | pH 8.4, 85°C, with crotonaldehyde | Thermococcus sp. | |
250 | - |
benzyl viologen | pH 8.4, 85°C, with crotonaldehyde | Thermococcus sp. | |
269 | - |
crotonaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
270 | - |
oxidized ferredoxin | ES-1 ferredoxin, pH 8.4, 85°C, with crotonaldehyde | Thermococcus sp. | |
272 | - |
isovalerylaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
343 | - |
acetaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
720 | - |
benzaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
950 | - |
formaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
960 | - |
phenylacetaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. | |
1100 | - |
propionaldehyde | pH 8.4, 85°C, benzyl viologen as electron acceptor | Thermococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay at | Thermococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | oxidation of acetaldehyde with benzyl viologen as electron acceptor | Thermococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Thermococcus sp. | |
form A cofactor | in presence of iodine | Thermococcus sp. | |
form B cofactor | in absence of iodine | Thermococcus sp. | |
molybdopterin | - |
Thermococcus sp. |