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Literature summary for 1.2.7.6 extracted from

  • Reher, M.; Gebhard, S.; Schoenheit, P.
    Glyceraldehyde-3-phosphate ferredoxin oxidoreductase (GAPOR) and nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN), key enzymes of the respective modified Embden-Meyerhof pathways in the hyperthermophilic crenarchaeota Pyrobaculum aerophi and Aeropyrum pernix (2007), FEMS Microbiol. Lett., 273, 196-205.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
maltose stimulates about 4fold Pyrobaculum aerophilum

Application

Application Comment Organism
degradation Pyrobaculum aerophilum contains a modified Embden-Meyerhof pathway, in which GAPOR replaces the GAPDH/phosphoglycerate kinase couple of the conventional Embden–Meyerhof pathway Pyrobaculum aerophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
D-Glyceraldehyde-3-phosphate
-
Pyrobaculum aerophilum
0.043
-
D-glyceraldehyde 3-phosphate pH 7.8, 50°C Pyrobaculum aerophilum
1
-
benzyl viologen
-
Pyrobaculum aerophilum
1
-
Oxidized benzyl viologen pH 7.8, 50°C Pyrobaculum aerophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
gel filtration Pyrobaculum aerophilum
65000
-
1 * 65000, SDS-PAGE Pyrobaculum aerophilum
66000
-
1 * 66000, SDS-PAGE Pyrobaculum aerophilum
70000
-
PAE1029, represents the GAPOR encoding gene gapor, sequence analysis Pyrobaculum aerophilum

Organism

Organism UniProt Comment Textmining
no activity in Aeropyrum pernix
-
-
-
Pyrobaculum aerophilum
-
-
-
Pyrobaculum aerophilum Q8ZXY6
-
-
Pyrobaculum aerophilum DSM 7523 Q8ZXY6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrobaculum aerophilum
by gel filtration, 113fold, to homogeneity Pyrobaculum aerophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.15
-
cell free extract, at 50°C Pyrobaculum aerophilum
0.375
-
at 50°C Pyrobaculum aerophilum
17
-
113fold purified, at 50°C, with a yield of 0.2% Pyrobaculum aerophilum
17
-
pH 7.8, 50°C Pyrobaculum aerophilum

Storage Stability

Storage Stability Organism
-20°C, Tris-HCl buffer, pH 9, several weeks Pyrobaculum aerophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + H2O + oxidized benzyl viologen
-
Pyrobaculum aerophilum 3-phospho-D-glycerate + H+ + reduced benzyl viologen
-
?
D-glyceraldehyde 3-phosphate + H2O + oxidized benzyl viologen
-
Pyrobaculum aerophilum DSM 7523 3-phospho-D-glycerate + H+ + reduced benzyl viologen
-
?
D-glyceraldehyde-3-phosphate + H2O + oxidized benzyl viologen is highly specific for glyceraldehyde-3-phosphate Pyrobaculum aerophilum 3-phospho-D-glycerate + H+ + reduced benzyl viologen
-
?
additional information does not use glyceraldehyde, acetaldehyde or formaldehyde as substrates Pyrobaculum aerophilum ?
-
?
additional information enzyme shows high specificity for glyceraldehyde-3-phosphate and does not use glyceraldehyde, acetaldehyde or formaldehyde Pyrobaculum aerophilum ?
-
?
additional information enzyme shows high specificity for glyceraldehyde-3-phosphate and does not use glyceraldehyde, acetaldehyde or formaldehyde Pyrobaculum aerophilum DSM 7523 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Pyrobaculum aerophilum
monomer 1 * 66000, SDS-PAGE Pyrobaculum aerophilum

Synonyms

Synonyms Comment Organism
aldehyde ferredoxin oxidoreductase
-
Pyrobaculum aerophilum
GAPOR
-
Pyrobaculum aerophilum
glyceraldehyde-3-phosphate ferredoxin oxidoreductase
-
Pyrobaculum aerophilum
PAE1029
-
Pyrobaculum aerophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
retains 70% of its activity upon incubation for 2 h (half-life, 500 min) Pyrobaculum aerophilum
100
-
2 h, 70% residual activity Pyrobaculum aerophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
-
Pyrobaculum aerophilum

pH Range

pH Minimum pH Maximum Comment Organism
7.7
-
60% of maximum activity Pyrobaculum aerophilum
8.3
-
60% of maximum activity Pyrobaculum aerophilum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.7 8.3 60% activity at pH 7.7 and 8.3 Pyrobaculum aerophilum

General Information

General Information Comment Organism
metabolism extracts of maltose/yeast extract/nitrate-grown cells contain all enzyme activities of a modified Embden-Meyerhof pathway, including ATP-dependent glucokinase, phosphoglucose isomerase, ATP-dependent 6-phosphofructokinase, fructose-1,6-phosphate aldolase, triose-phosphate isomerase, glyceraldehyde-3-phosphate ferredoxin oxidoreductase, phosphoglycerate mutase, enolase and pyruvate kinase Pyrobaculum aerophilum