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Literature summary for 1.2.7.7 extracted from

  • Heider, J.; Mai, X.; Adams, M.W.
    Characterization of 2-ketoisovalerate ferredoxin oxidoreductase, a new and reversible coenzyme A-dependent enzyme involved in peptide fermentation by hyperthermophilic archaea (1996), J. Bacteriol., 178, 780-787.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.7.7

Activating Compound

Activating Compound Comment Organism Structure
thiamine diphosphate enzyme contains 0.17 mol per 230000 g of protein Thermococcus litoralis
thiamine diphosphate Km 0.076 mM Pyrococcus sp.
thiamine diphosphate Km 0.3 mM Thermococcus litoralis
thiamine diphosphate thiamine diphosphate can be replaced by thiamine or thiamine monophosphate Thermococcus litoralis
thiamine diphosphate requires for maximal activity of enzyme Pyrococcus sp.
thiamine diphosphate requires for maximal activity of enzyme Pyrococcus furiosus
thiamine diphosphate requires for maximal activity of enzyme Thermococcus litoralis

Inhibitors

Inhibitors Comment Organism Structure
EDTA 80°C, complete loss of activity Thermococcus litoralis
iodoacetate 23°C, in a concentration-dependent manner Thermococcus litoralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
 CoA pH 6.9, 85°C, under argon Pyrococcus sp.
0.012
-
 2-keto-3-methylvalerate pH 6.9, 85°C, under argon Thermococcus litoralis
0.017
-
 oxidized ferredoxin pH 6.9, 85°C, under argon Thermococcus litoralis
0.026
-
 2-ketoisocaproate pH 6.9, 85°C, under argon Thermococcus litoralis
0.048
-
 CO2 pH 7.0, 85°C, under argon Thermococcus litoralis
0.049
-
 2-ketocaproate pH 6.9, 85°C, under argon Thermococcus litoralis
0.05
-
 CoA pH 6.9, 85°C, under argon Thermococcus litoralis
0.058
-
 4-methylthio-2-oxobutanoate pH 6.9, 85°C, under argon Thermococcus litoralis
0.094
-
 2-ketoisovalerate pH 6.9, 85°C, under argon Thermococcus litoralis
0.11
-
 2-ketoisovalerate pH 6.9, 85°C, under argon Pyrococcus sp.
0.15
-
 2-keto-3-methylvalerate pH 6.9, 85°C, under argon Pyrococcus sp.
0.17
-
 2-Ketobutyrate pH 6.9, 85°C, under argon Pyrococcus sp.
0.176
-
 2-ketoisocaproate pH 6.9, 85°C, under argon Pyrococcus sp.
0.22
-
 phenylpyruvate pH 6.9, 85°C, under argon Thermococcus litoralis
0.25
-
 isobutyryl-CoA pH 7.0, 85°C, under argon Thermococcus litoralis
0.276
-
 3-methyl-2-oxobutanoate pH 6.9, 85°C, under argon Pyrococcus sp.
0.311
-
 2-ketocaproate pH 6.9, 85°C, under argon Pyrococcus sp.
0.38
-
 2-Ketobutyrate pH 6.9, 85°C, under argon Thermococcus litoralis
0.466
-
 phenylglyoxylate pH 6.9, 85°C, under argon Pyrococcus sp.
0.6
-
 phenylglyoxalate pH 6.9, 85°C, under argon Thermococcus litoralis
0.75
-
 pyruvate pH 6.9, 85°C, under argon Thermococcus litoralis
2.8
-
 pyruvate pH 6.9, 85°C, under argon Pyrococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 11 iron atoms per heterotetramer and two types of FeS are evident Thermococcus litoralis
Mg required for maximal activity of enzyme Pyrococcus sp.
Mg required for maximal activity of enzyme Thermococcus litoralis
Mg Km 3.00 mM Thermococcus litoralis
Mg Km 0.89 mM Pyrococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
 alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Pyrococcus sp.
13000
-
 alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
23000
-
 alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Pyrococcus sp.
23000
-
 alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
34000
-
 alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Pyrococcus sp.
34000
-
 alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
47000
-
 alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Pyrococcus sp.
47000
-
 alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
230000
-
 calculated from amino acid sequence Thermococcus litoralis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermococcus sp.
-
 ?
-
 ?
additional information Pyrococcus furiosus enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids ?
-
 ?
additional information Thermococcus litoralis enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids ?
-
 ?
additional information Thermococcus litoralis enzyme functions in the biosynthesis of branched-chain amino acids from volatile fatty acids that are excreted under nutrient-sufficient conditions ?
-
 ?
additional information Thermococcus sp. ES-1
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
 DSM 3638
-
Pyrococcus sp.
-
 ES-4
-
Pyrococcus sp. ES-4
-
 ES-4
-
Thermococcus litoralis
-
-
-
Thermococcus sp.
-
 ES-1
-
Thermococcus sp. ES-1
-
 ES-1
-

Oxidation Stability

Oxidation Stability Organism
about 50% of the enzyme activity in the cell extract is lost after exposure to air for 2 min, no activity loss if the extract is under argon Thermococcus litoralis

Purification (Commentary)

Purification (Comment) Organism
under anaerobic conditions Pyrococcus sp.
under anaerobic conditions Pyrococcus furiosus
under anaerobic conditions Thermococcus sp.
under anaerobic conditions Thermococcus litoralis

Reaction

Reaction Comment Organism Reaction ID
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Pyrococcus sp.
a
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Pyrococcus furiosus
a
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Thermococcus sp.
a
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Thermococcus litoralis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
 Kommentar Thermococcus litoralis
46
-
-
 Thermococcus litoralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-keto-3-methylvalerate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-keto-3-methylvalerate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-keto-3-methylvalerate + CoA + oxidized ferredoxin
-
 Thermococcus sp. 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-keto-3-methylvalerate + CoA + oxidized ferredoxin
-
 Thermococcus litoralis 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-keto-3-methylvalerate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. ES-4 2-methylbutanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketobutyrate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. propanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketobutyrate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus propanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketobutyrate + CoA + oxidized ferredoxin
-
 Thermococcus sp. propanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketobutyrate + CoA + oxidized ferredoxin
-
 Thermococcus litoralis propanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketocaproate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. pentanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketocaproate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus pentanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketocaproate + CoA + oxidized ferredoxin
-
 Thermococcus sp. pentanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketocaproate + CoA + oxidized ferredoxin
-
 Thermococcus litoralis pentanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketoisocaproate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketoisocaproate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketoisocaproate + CoA + oxidized ferredoxin
-
 Thermococcus sp. S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketoisocaproate + CoA + oxidized ferredoxin
-
 Thermococcus litoralis S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketoisovalerate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketoisovalerate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketoisovalerate + CoA + oxidized ferredoxin
-
 Thermococcus sp. S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketoisovalerate + CoA + oxidized ferredoxin or benzyl viologen or methyl viologen as electron acceptor Thermococcus litoralis S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketoisovalerate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. ES-4 S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
 ?
2-ketoisovalerate + CoA + oxidized ferredoxin
-
 Thermococcus sp. ES-1 S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
 ?
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin
-
 Pyrococcus sp. S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+
-
 r
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin
-
 Thermococcus litoralis S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+
-
 r
4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin
-
 Thermococcus sp. 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin
-
 Thermococcus litoralis 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
glyoxylate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. formyl-CoA + CO2 + reduced ferredoxin
-
 ?
glyoxylate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus formyl-CoA + CO2 + reduced ferredoxin
-
 ?
glyoxylate + CoA + oxidized ferredoxin
-
 Thermococcus sp. formyl-CoA + CO2 + reduced ferredoxin
-
 ?
glyoxylate + CoA + oxidized ferredoxin
-
 Thermococcus litoralis formyl-CoA + CO2 + reduced ferredoxin
-
 ?
hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
 ?
hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
 ?
hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
 Thermococcus sp. 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
 ?
hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
 Thermococcus litoralis 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
 ?
indolpyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. indolacetyl-CoA + CO2 + reduced ferredoxin
-
 ?
indolpyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus indolacetyl-CoA + CO2 + reduced ferredoxin
-
 ?
indolpyruvate + CoA + oxidized ferredoxin
-
 Thermococcus sp. indolacetyl-CoA + CO2 + reduced ferredoxin
-
 ?
indolpyruvate + CoA + oxidized ferredoxin
-
 Thermococcus litoralis indolacetyl-CoA + CO2 + reduced ferredoxin
-
 ?
isobutyryl-CoA + CO2 + reduced benzyl viologen
-
 Pyrococcus sp. 2-oxoisopentanoate + CoA + benzyl viologen
-
 ?
isobutyryl-CoA + CO2 + reduced benzyl viologen
-
 Pyrococcus furiosus 2-oxoisopentanoate + CoA + benzyl viologen
-
 ?
isobutyryl-CoA + CO2 + reduced benzyl viologen
-
 Thermococcus sp. 2-oxoisopentanoate + CoA + benzyl viologen
-
 ?
isobutyryl-CoA + CO2 + reduced benzyl viologen
-
 Thermococcus litoralis 2-oxoisopentanoate + CoA + benzyl viologen
-
 ?
additional information
-
 Thermococcus sp. ?
-
 ?
additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Pyrococcus sp. ?
-
 ?
additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Pyrococcus furiosus ?
-
 ?
additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Thermococcus sp. ?
-
 ?
additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Thermococcus litoralis ?
-
 ?
additional information no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate Pyrococcus sp. ?
-
 ?
additional information no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate Pyrococcus furiosus ?
-
 ?
additional information no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate Thermococcus sp. ?
-
 ?
additional information no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate Thermococcus litoralis ?
-
 ?
additional information enzyme functions in the biosynthesis of branched-chain amino acids from volatile fatty acids that are excreted under nutrient-sufficient conditions Thermococcus litoralis ?
-
 ?
additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Pyrococcus sp. ES-4 ?
-
 ?
additional information no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate Pyrococcus sp. ES-4 ?
-
 ?
additional information
-
 Thermococcus sp. ES-1 ?
-
 ?
additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Thermococcus sp. ES-1 ?
-
 ?
additional information no substrate are 2-ketoglutarate, 2-ketomalonate, imidazolpyruvate Thermococcus sp. ES-1 ?
-
 ?
phenylglyoxylate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. benzoyl-CoA + CO2 + reduced ferredoxin
-
 ?
phenylglyoxylate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus benzoyl-CoA + CO2 + reduced ferredoxin
-
 ?
phenylglyoxylate + CoA + oxidized ferredoxin
-
 Thermococcus sp. benzoyl-CoA + CO2 + reduced ferredoxin
-
 ?
phenylglyoxylate + CoA + oxidized ferredoxin
-
 Thermococcus litoralis benzoyl-CoA + CO2 + reduced ferredoxin
-
 ?
phenylpyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. phenylacetyl-CoA + CO2 + reduced ferredoxin
-
 ?
phenylpyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
 ?
phenylpyruvate + CoA + oxidized ferredoxin
-
 Thermococcus sp. phenylacetyl-CoA + CO2 + reduced ferredoxin
-
 ?
phenylpyruvate + CoA + oxidized ferredoxin
-
 Thermococcus litoralis phenylacetyl-CoA + CO2 + reduced ferredoxin
-
 ?
pyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. acetyl-CoA + CO2 + reduced ferredoxin
-
 ?
pyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus acetyl-CoA + CO2 + reduced ferredoxin
-
 ?
pyruvate + CoA + oxidized ferredoxin
-
 Thermococcus sp. acetyl-CoA + CO2 + reduced ferredoxin
-
 ?
pyruvate + CoA + oxidized ferredoxin
-
 Thermococcus litoralis acetyl-CoA + CO2 + reduced ferredoxin
-
 ?
pyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus sp. ES-4 acetyl-CoA + CO2 + reduced ferredoxin
-
 ?
pyruvate + CoA + oxidized ferredoxin
-
 Thermococcus sp. ES-1 acetyl-CoA + CO2 + reduced ferredoxin
-
 ?

Subunits

Subunits Comment Organism
octamer alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Pyrococcus sp.
octamer alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
 ketoisovalerate oxidation Thermococcus litoralis
98
-
 ketoisovalerate oxidation Pyrococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20
-
 enzyme is inactive at 20°C Pyrococcus sp.
20
-
 enzyme is inactive at 20°C Thermococcus litoralis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 pH 8.0, Tris-HCl, half-life 3 h Thermococcus litoralis
95
-
 pH 8.0, Tris-HCl, half-life 50 min Thermococcus litoralis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0133
-
 glyoxylate pH 6.9, 85°C, under argon Thermococcus litoralis
0.267
-
 pyruvate pH 6.9, 85°C, under argon Thermococcus litoralis
0.433
-
 indolpyruvate pH 6.9, 85°C, under argon Thermococcus litoralis
0.467
-
 phenylpyruvate pH 6.9, 85°C, under argon Pyrococcus sp.
0.6
-
 phenylpyruvate pH 6.9, 85°C, under argon Thermococcus litoralis
0.667
-
 4-methylthio-2-oxobutanoate pH 6.9, 85°C, under argon Thermococcus litoralis
0.833
-
 hydroxyphenylpyruvate pH 6.9, 85°C, under argon Thermococcus litoralis
0.833
-
 phenylglyoxalate pH 6.9, 85°C, under argon Thermococcus litoralis
0.933
-
 2-ketocaproate pH 6.9, 85°C, under argon Pyrococcus sp.
1
-
 2-ketocaproate pH 6.9, 85°C, under argon Thermococcus litoralis
1.07
-
 3-methyl-2-oxobutanoate pH 6.9, 85°C, under argon Pyrococcus sp.
1.27
-
 2-keto-3-methylvalerate pH 6.9, 85°C, under argon Thermococcus litoralis
1.33
-
 phenylglyoxylate pH 6.9, 85°C, under argon Pyrococcus sp.
1.47
-
 2-Ketobutyrate pH 6.9, 85°C, under argon Pyrococcus sp.
1.53
-
 oxidized ferredoxin pH 6.9, 85°C, under argon Thermococcus litoralis
1.73
-
 pyruvate pH 6.9, 85°C, under argon Pyrococcus sp.
2
-
 2-ketoisocaproate pH 6.9, 85°C, under argon Thermococcus litoralis
2.07
-
 2-keto-3-methylvalerate pH 6.9, 85°C, under argon Pyrococcus sp.
2.47
-
 2-ketoisocaproate pH 6.9, 85°C, under argon Pyrococcus sp.
3
-
 2-ketoisovalerate pH 6.9, 85°C, under argon Thermococcus litoralis
3.67
-
 2-ketoisovalerate pH 6.9, 85°C, under argon Pyrococcus sp.
5
-
 CoA pH 6.9, 85°C, under argon Thermococcus litoralis
5.6
-
 CoA pH 6.9, 85°C, under argon Pyrococcus sp.
22.3
-
 2-Ketobutyrate pH 6.9, 85°C, under argon Thermococcus litoralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermococcus litoralis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 pH 6.0: less than 50% loss of activity, pH 8.0: 25% loss of activity Thermococcus litoralis