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Literature summary for 1.2.7.8 extracted from
- Indolepyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus. A new enzyme involved in peptide fermentation (1994), J. Biol. Chem., 269, 16726-16732.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | activity increases about 8fold, Km for thiamine diphosphate 0.004 mM | Pyrococcus furiosus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Pyrococcus furiosus | |
| cyanide | - |
Pyrococcus furiosus | |
| KCN | - |
Pyrococcus furiosus | |
| additional information | not inhibited by CO, unaffected by sodium nitrite, not inhibited by sodium fluoride or sodium azide, sodium benzoate, phenylacetate, pyruvate, 2-ketoglutarate and oxamate have no effect on activity | Pyrococcus furiosus | |
| Zn2+ | - |
Pyrococcus furiosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
CoA | pH 8.4, 80°C, substrate indolepyruvate | Pyrococcus furiosus | |
| 0.021 | - |
CoA | pH 8.4, 80°C, substrate phenylpyruvate | Pyrococcus furiosus | |
| 0.048 | - |
oxidized ferredoxin | pH 8.4, 80°C | Pyrococcus furiosus | |
| 0.095 | - |
phenylpyruvate | pH 8.4, 80°C | Pyrococcus furiosus | |
| 0.11 | - |
p-hydroxyphenylpyruvate | pH 8.4, 80°C | Pyrococcus furiosus | |
| 0.25 | - |
Indolepyruvate | pH 8.4, 80°C | Pyrococcus furiosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
| 66000 | - |
2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
| 180000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | Pyrococcus furiosus | involved in peptide fermentation and metabolism of aromatic amino acids | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
DSM 3638 | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| enzyme is sensitive to inactivation by O2, losing 50% of its activity after exposure to air for 20 min at 23°C | Pyrococcus furiosus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 38 | - |
- |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
| (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | involved in peptide fermentation and metabolism of aromatic amino acids | Pyrococcus furiosus | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
| (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | S-2-(indol-3-yl)acetyl-CoA + CO2 + 2 reduced ferredoxin + H+ | - |
r | |
| 2-keto-4-methylthiobutyrate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | CO2 + reduced ferredoxin + 3-methylthiopropionyl-CoA | - |
r | |
| 2-ketoisocaproate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | CO2 + reduced ferredoxin + isovaleryl-CoA | - |
r | |
| additional information | pyruvate and transaminated forms of the amino acids aspartate, valine, glutamate and alanine, 2-ketobutyrate, oxalacetate, 2-ketoisovalerate, 2-ketoglutarate, 2-ketomalonate and phenylglyoxylate are not oxidized | Pyrococcus furiosus | ? | - |
? | |
| p-hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | p-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
| phenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
exhibits optimal activity above at pH 8.0 | Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 90 | shows dramatic increase in activity above 70°C | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
virtually inactive at 25°C, quite thermostable, with a half-life of activity at 80°C of about 80 min under anaerobic conditions | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 10.5 | at 80°C | Pyrococcus furiosus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | not active at pH 5.2 and pH 11.0 | Pyrococcus furiosus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.5 | - |
KCN | pH 8.4, 80°C | Pyrococcus furiosus | |
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