Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sulfide | activated up to fivefold by treatment with sulfide under reducing conditions | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycerol | 60% v/v, 6 h, 60% loss of activity | Pyrococcus furiosus | |
Glyoxal | above 1 mM | Pyrococcus furiosus | |
additional information | the enzyme is not inhibited when it is incubated for up to 24 h with iodoacetate, arsenite, or cyanide (each at a concentration of 5 mM) prior to being assayed under standard conditions in the absence of these reagents | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
oxidized ferredoxin | 80°C, pH 8.4 | Pyrococcus furiosus | |
0.8 | - |
glutaric dialdehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
8 | - |
Succinic semialdehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
15 | - |
phenylpropionaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
25 | - |
formaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
25 | - |
Indole-3-acetaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
60 | - |
acetaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
62 | - |
propionaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Calcium | contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit | Pyrococcus furiosus | |
Iron | contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit | Pyrococcus furiosus | |
Tungsten | tungsten-containing enzyme. The enzyme contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68700 | - |
4 * 68700, calculated from sequence | Pyrococcus furiosus |
275000 | - |
gel filtration | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | O93738 | - |
- |
Oxidation Stability | Organism |
---|---|
enzyme as purified under standard conditions is oxygen sensitive, with the t50% being about 12 h when the enzyme is exposed to air. No activity is lost under anaerobic conditions | Pyrococcus furiosus |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
58 | - |
pH 8.4, 90°C | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + H2O + oxidized benzyl viologen | - |
Pyrococcus furiosus | acetate + reduced benzyl viologen + H+ | - |
? | |
formaldehyde + H2O + 2 oxidized ferredoxin | formaldehyde is unlikely to be the physiologiocal substrate (Km-value: 25 mM) | Pyrococcus furiosus | formate + 2 reduced ferredoxin + 2 H+ | - |
? | |
formaldehyde + H2O + oxidized benzyl viologen | - |
Pyrococcus furiosus | formate + reduced benzyl viologen + H+ | - |
? | |
glutaric dialdehyde + H2O + oxidized benzyl viologen | best substrate, C5- or C6-type aldehydes might be physiologically relevant substrates | Pyrococcus furiosus | ? + reduced benzyl viologen + H+ | - |
? | |
indole-3-acetaldehyde + H2O + oxidized benzyl viologen | - |
Pyrococcus furiosus | indole-3-acetate + reduced benzyl viologen + H+ | - |
? | |
additional information | low activity with butyraldehyde, crotonaldehyde, no activity with: isovaleraldehyde, benzaldehyde, salicaldehyde, 2-furfuraldehyde. It does not oxidize lactate, 2-hydroxybutyrate, 2-hydroxyvalerate, 2-hydroxycaproate, or 1-ethyl-2-hydroxycaproate and it does not reduce pyruvate, 2-oxobutyrate, 2-oxovalerate, 1-ethyl-3-oxovalerate, 1-ethyl-4-oxovalerate, or 2-oxocaproate | Pyrococcus furiosus | ? | - |
? | |
phenylpropionaldehyde + H2O + oxidized benzyl viologen | - |
Pyrococcus furiosus | phenylpropionate + reduced benzyl viologen + H+ | - |
? | |
propionaldehyde + H2O + oxidized benzyl viologen | - |
Pyrococcus furiosus | propionate + reduced benzyl viologen + H+ | - |
? | |
succinic semialdehyde + H2O + oxidized benzyl viologen | - |
Pyrococcus furiosus | succinate + reduced benzyl viologen + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 68700, calculated from sequence | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
FOR | - |
Pyrococcus furiosus |
formaldehyde ferredoxin oxidoreductase | - |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
8 h, 50% loss of activity | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2300 | - |
Indole-3-acetaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
5700 | - |
Succinic semialdehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
11000 | - |
propionaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
25000 | - |
phenylpropionaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
42000 | - |
glutaric dialdehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
44000 | - |
acetaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
71000 | - |
formaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
the activity of the pure enzyme with benzyl viologen as the electron acceptor increases linearly with pH over the pH range 5.5 (1.5 U/mg) to 10.0 (55 U/mg) at 80°C | Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | the activity of the pure enzyme with benzyl viologen as the electron acceptor increases linearly with pH over the pH range 5.5 (1.5 U/mg) to 10.0 (55 U/mg) at 80°C | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme contains a pterin cofactor | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
90 | - |
Indole-3-acetaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
170 | - |
propionaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
710 | - |
Succinic semialdehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
730 | - |
acetaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
1700 | - |
phenylpropionaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
3000 | - |
formaldehyde | 80°C, pH 8.4 | Pyrococcus furiosus | |
60000 | - |
glutaric dialdehyde | 80°C, pH 8.4 | Pyrococcus furiosus |