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Literature summary for 1.2.99.6 extracted from

  • Huber, C.; Skopan, H.; Feicht, R.; White, H.; Simon, H.
    Pterin cofactor, substrate specificity, and observations on the kinetics of the reversible tungsten-containing aldehyde oxidoreductase from Clostridium thermoaceticum (1995), Arch. Microbiol., 164, 110-118.
No PubMed abstract available
Search for more literature for 1.2.99.6

Inhibitors

Inhibitors Comment Organism Structure
2-Hydroxypyridine-5-carboxylate
-
 Moorella thermoacetica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 4-methoxybenzaldehyde terephthalaldehyde, with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
0.005
-
 4-methoxybenzaldehyde with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
0.006
-
 Butanal with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
0.008
-
 propanal with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
0.009
-
 benzaldehyde with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
0.01
-
 ES-1 ferredoxin acetaldehyde, with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
0.012
-
 cinnamaldehyde with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
0.02
-
 benzaldehyde 3,4-dimethoxy-5-hydroxybenzaldehyde, with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
0.03
-
 reduced carbamoyl methyl viologen with propanoate as cosubstrate Moorella thermoacetica
0.05
-
 Indoleacetaldehyde reduced carbamoyl methyl viologen, with benzoate as cosubstrate Moorella thermoacetica
0.1
-
 5-Phenyl-2,4-pentadienate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
0.16
-
 reduced carbamoyl methyl viologen with benzoate as cosubstrate Moorella thermoacetica
1
-
 4-Chlorobenzoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.6
-
 methanal with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.6
-
 methanal 4-fluorobenzoate, with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.6
-
 methanal 4-methoxybenzoate, with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
1.7
-
 cinnamate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
2.4
-
 3-chlorobenzoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
2.9
-
 methyl viologen 3-methoxybenzoate, with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
3
-
 4-hydroxy-3-methoxybenzaldehyde with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
3
-
 4-hydroxy-3-methoxybenzaldehyde 4-methylbenzoate, with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
3.5
-
 3-Fluorobenzoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
4
-
 terephthalate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
5.6
-
 acetate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
6.1
-
 3-methylbenzoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
6.3
-
 propanoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
8
-
 4-(hydroxymethyl)benzoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
10
-
 3,5-dimethoxy-4-hydroxybenzaldehyde with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
12
-
 4-hydroxybenzaldehyde with carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
31
-
 benzoate with reduced carbamoyl methyl viologen as cosubstrate, pH 4.5 Moorella thermoacetica
53
-
 benzoate with reduced carbamoyl methyl viologen as cosubstrate, pH 5.5 Moorella thermoacetica
78
-
 3-hydroxybenzoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
78
-
 3-hydroxybenzoate pyridine-4-carboxylate, with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica
130
-
 4-hydroxybenzoate with reduced carbamoyl methyl viologen as cosubstrate Moorella thermoacetica

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-Dimethoxy-5-hydroxy-benzaldehyde + carbamoyl methyl viologen
-
 Moorella thermoacetica 3,4-Dimethoxy-5-hydroxy-benzoate + reduced carbamoyl methyl viologen
-
 ?
3,5-Dimethoxy-4-hydroxy-benzaldehyde + carbamoyl methyl viologen
-
 Moorella thermoacetica 3,5-Dimethoxy-4-hydroxy-benzoate + reduced carbamoyl methyl viologen
-
 ?
3-Chlorobenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 3-Chlorobenzaldehyde + carbamoyl methyl viologen
-
 ?
3-Fluorobenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 3-Fluorobenzaldehyde + carbamoyl methyl viologen
-
 ?
3-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 3-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
 ?
3-Methoxybenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 3-Methoxybenzaldhyde + carbamoyl methyl viologen
-
 ?
3-Methylbenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 3-Methylbenzaldehyde + carbamoyl methyl viologen
-
 ?
4-(Hydroxymethyl)benzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 4-(Hydroxymethyl)benzaldehyde + carbamoyl methyl viologen
-
 ?
4-Chlorobenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 4-Chlorobenzaldehyde + carbamoyl methyl viologen
-
 ?
4-Fluorobenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 4-Fluorobenzaldehyde + carbamoyl methyl viologen
-
 ?
4-Hydroxy-3-methoxy-benzaldehyde + carbamoyl methyl viologen
-
 Moorella thermoacetica 4-Hydroxy-3-methoxy-benzoate + reduced carbamoyl methyl viologen
-
 ?
4-Hydroxybenzaldehyde + carbamoyl methyl viologen r Moorella thermoacetica 4-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
 ?
4-Methoxybenzaldehyde + benzyl viologen
-
 Moorella thermoacetica 4-Methoxybenzoate + reduced carbamoyl methyl viologen
-
 ?
4-Methylbenzoate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica 4-Methylbenzaldehyde + carbamoyl methyl viologen
-
 ?
5-Phenyl-2,4-pentadienate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica ? + carbamoyl methyl viologen
-
 ?
Acetaldehyde + carbamoyl methyl viologen r Moorella thermoacetica acetate + reduced carbamoyl methyl viologen
-
 ?
Benzaldehyde + carbamoyl methyl viologen r Moorella thermoacetica Benzoate + reduced carbamoyl methyl viologen
-
 ?
Butanal + NH2CO-methyl viologen
-
 Moorella thermoacetica Butanoate + reduced NH2CO-methyl viologen
-
 ?
Cinnamaldehyde + carbamoyl methyl viologen r Moorella thermoacetica Cinnamate + reduced carbamoyl methyl viologen
-
 ?
Indoleacetaldehyde + benzyl viologen
-
 Moorella thermoacetica Indoleacetate + reduced benzyl viologen
-
 ?
Methanal + carbamoyl methyl viologen
-
 Moorella thermoacetica Formate + reduced methyl viologen
-
 ?
Propanal + methyl viologen
-
 Moorella thermoacetica Propanoate + reduced methyl viologen
-
 ?
Propanal + NH2CO-methyl viologen r Moorella thermoacetica Propanoate + reduced carbamoyl methyl viologen
-
 ?
Pyridine 4-carboxylate + reduced carbamoyl methyl viologen
-
 Moorella thermoacetica Pyridine 4-carboxaldehyde + carbamoyl methyl viologen
-
 ?
terephthalaldehyde + carbamoyl methyl viologen r Moorella thermoacetica terephthalate + reduced carbamoyl methyl viologen
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
46
-
 at pH 4.5 Moorella thermoacetica
51
-
 propanoate as substrate, pH 4.8 Moorella thermoacetica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
51 71 51°C: optimum, 71°C: about 60% of maximal activity, propanoate and reduced carbamoyl methyl viologen as substrates Moorella thermoacetica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1
-
 4-fluorobenzoate Moorella thermoacetica
4.4 4.7 benzoate and reduced carbamoyl methyl viologen as substrate Moorella thermoacetica
4.7
-
 propanoate and reduced carbamoyl methyl viologen as substrate Moorella thermoacetica
4.7
-
 4-methoxybenzoate Moorella thermoacetica
4.7
-
 4-methoxybenzoate and reduced carbamoyl methyl viologen as substrate Moorella thermoacetica

pH Range

pH Minimum pH Maximum Comment Organism
3.9 4.1 pH 3.9: 80% of maximal activity, pH 4.1: optimum, 4-fluorobenzoate and reduced carbamoyl methyl viologen as substrates Moorella thermoacetica
3.9 4.7 pH 3.9: 55% of maximal activity, pH 4.7: optimum, 4-methoxybenzoate and reduced carbamoyl methyl viologen as substrates Moorella thermoacetica
3.9 4.7 pH 3.9: 55% of maximal activity, pH 4.3-4.7: optimum Moorella thermoacetica
4.7 5.5 pH 4.7: optimum, pH 5.5: 20% of maximal activity, propanoate reduced carbamoyl methyl viologen as substrates Moorella thermoacetica