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Literature summary for 1.2.99.7 extracted from
- Purification and characterization of a benzylviologen-linked tungsten-containing aldehyde oxidoreductase from Desulfovibrio gigas (1995), J. Bacteriol., 177, 6195-6200.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenite | 5 mM, complete inhibition | Megalodesulfovibrio gigas |  |
| iodoacetate | 5 mM, complete inhibition | Megalodesulfovibrio gigas | |
| KCN | 5 mM, 64% inhibition | Megalodesulfovibrio gigas | |
| methanol | 1 M, 62% inhibition | Megalodesulfovibrio gigas | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.55 | - |
benzylviologen | 30°C, pH 7.5 | Megalodesulfovibrio gigas | |
| 10.8 | - |
propionaldehyde | 30°C, pH 7.5 | Megalodesulfovibrio gigas | |
| 12.5 | - |
acetaldehyde | 30°C, pH 7.5 | Megalodesulfovibrio gigas | |
| 20 | - |
benzaldehyde | 30°C, pH 7.5 | Megalodesulfovibrio gigas | |
| 30.8 | - |
furfural | 30°C, pH 7.5 | Megalodesulfovibrio gigas | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 4.8 iron per subunit, probably a [Fe-4S]1+ cluster | Megalodesulfovibrio gigas | |
| K+ | 10 mM, 50% activation | Megalodesulfovibrio gigas | |
| Tungsten | 0.68 tungsten per subunit | Megalodesulfovibrio gigas | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
2 * 62000, SDS-PAGE | Megalodesulfovibrio gigas |
| 120000 | - |
gelfiltration, Sephacryl S-300 | Megalodesulfovibrio gigas |
| 126000 | - |
gel filtration, Superose 12 HR | Megalodesulfovibrio gigas |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Megalodesulfovibrio gigas | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| exposure to air leads to inactivation | Megalodesulfovibrio gigas |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose, Butyl-Sepharose, DEAE-memsep 1010 | Megalodesulfovibrio gigas |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 38.5 | - |
- |
Megalodesulfovibrio gigas |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + H2O + benzylviologen | natural electron aceptor not yet known, the artificial electron acceptors benzylviologen, methylviologen, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl) and 2,6-dichlorphenol-indophenol have relative activities of 100%, 19%, 5%, and 1.3%, respectively | Megalodesulfovibrio gigas | acetate + reduced benzylviologen | - |
? | |
| benzaldehyde + H2O + benzylviologen | 16% of activity with acetaldehyde | Megalodesulfovibrio gigas | benzoate + reduced benzylviologen | - |
? | |
| benzaldehyde + H2O + benzylviologen | 93% of activity with acetaldehyde | Megalodesulfovibrio gigas | benzoate + reduced benzylviologen | - |
? | |
| butyraldehyde + H2O + benzylviologen | 93% of activity with acetaldehyde | Megalodesulfovibrio gigas | butanoate + reduced benzylviologen | - |
? | |
| formaldehyde + H2O + benzylviologen | 16% of activity with acetaldehyde | Megalodesulfovibrio gigas | formate + reduced benzylviologen | - |
? | |
| furfural + H2O + benzylviologen | 77% of activity with acetaldehyde | Megalodesulfovibrio gigas | furfurate + reduced benzylviologen | - |
? | |
| glutaraldehyde + H2O + benzylviologen | 20% of activity with acetaldehyde | Megalodesulfovibrio gigas | glutarate + reduced benzylviologen | - |
? | |
| glycoaldehyde + H2O + benzylviologen | 23% of activity with acetaldehyde | Megalodesulfovibrio gigas | glycolate + reduced benzylviologen | - |
? | |
| hexanal + H2O + benzylviologen | 6% of activity with acetaldehyde | Megalodesulfovibrio gigas | hexanoate + reduced benzylviologen | - |
? | |
| octanal + H2O + benzylviologen | 93% of activity with acetaldehyde | Megalodesulfovibrio gigas | octanoate + reduced benzylviologen | - |
? | |
| p-anisaldehyde + H2O + benzylviologen | 5% of activity with acetaldehyde | Megalodesulfovibrio gigas | p-anisic acid + reduced benzylviologen | - |
? | |
| pentanal + H2O + benzylviologen | 9% of activity with acetaldehyde | Megalodesulfovibrio gigas | pentanoate + reduced benzylviologen | - |
? | |
| phenylacetaldehyde + H2O + benzylviologen | 31% of activity with acetaldehyde | Megalodesulfovibrio gigas | phenylacetate + reduced benzylviologen | - |
? | |
| propionaldehyde + H2O + benzylviologen | 117% of activity with acetaldehyde | Megalodesulfovibrio gigas | propionate + reduced benzylviologen | - |
? | |
| salicylaldehyde + H2O + benzylviologen | 14% of activity with acetaldehyde | Megalodesulfovibrio gigas | salicylate + reduced benzylviologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 62000, SDS-PAGE | Megalodesulfovibrio gigas |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BV-AIDH | - |
Megalodesulfovibrio gigas |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | - |
Megalodesulfovibrio gigas |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
broad optimum | Megalodesulfovibrio gigas |
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