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Literature summary for 1.20.1.1 extracted from

  • Johannes, T.W.; Woodyer, R.D.; Zhao, H.
    Directed evolution of a thermostable phosphite dehydrogenase for NAD(P)H regeneration (2005), Appl. Environ. Microbiol., 71, 5728-5734.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.20.1.1

Application

Application Comment Organism
biotechnology evaluation of enzyme mutants for regeneration of reduced cofactors NADH and NADPH in industrial processes and comparison with Candida boidinii formate dehydrogenase Pseudomonas stutzeri

Protein Variants

Protein Variants Comment Organism
I150F thermostable mutant, half-life at 45°C 7.0 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q132R thermostable mutant, half-life at 45°C 2.3 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q132R/Q137R/I150F/Q215L/R275Q thermostable mutant, half-life at 45°C 161 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q137R thermostable mutant, half-life at 45°C 3.8 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q137R/I150F/Q215L/R275Q thermostable mutant, half-life at 45°C 200 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q137R/I150F/Q215L/R275Q/A319E thermostable mutant, half-life at 45°C 567 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q137R/I150F/Q215L/R275Q/D162N/V315A thermostable mutant, half-life at 45°C 614 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q137R/I150F/Q215L/R275Q/L276Q thermostable mutant, half-life at 45°C 437 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A thermostable mutant, half-life at 45°C 1421 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V thermostable mutant, half-life at 45°C 2315 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K thermostable mutant, half-life at 45°C 1515 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L thermostable mutant, half-life at 45°C 1765 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R thermostable mutant, half-life at 45°C 2350 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V thermostable mutant, half-life at 45°C 8440 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I thermostable mutant, half-life at 45°C 2000 min compared to 1.4 min of wild-type Pseudomonas stutzeri
Q215L thermostable mutant, half-life at 45°C 8.7 min compared to 1.4 min of wild-type Pseudomonas stutzeri
R275Q thermostable mutant, half-life at 45°C 4.6 min compared to 1.4 min of wild-type Pseudomonas stutzeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V, pH 7.3, 25°C Pseudomonas stutzeri
0.045
-
 phosphite mutant Q132R, pH 7.3, 25°C Pseudomonas stutzeri
0.046
-
 NAD+ mutant Q132R/Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C Pseudomonas stutzeri
0.046
-
 phosphite mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V, pH 7.3, 25°C Pseudomonas stutzeri
0.048
-
 phosphite mutant Q137R, pH 7.3, 25°C Pseudomonas stutzeri
0.05
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V, pH 7.3, 25°C Pseudomonas stutzeri
0.052
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/A319E, pH 7.3, 25°C Pseudomonas stutzeri
0.056
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q, pH 7.3, 25°C Pseudomonas stutzeri
0.057
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R, pH 7.3, 25°C Pseudomonas stutzeri
0.057
-
 phosphite wild-type, pH 7.3, 25°C Pseudomonas stutzeri
0.058
-
 phosphite mutant Q215L, pH 7.3, 25°C Pseudomonas stutzeri
0.06
-
 NAD+ mutant Q132R, pH 7.3, 25°C Pseudomonas stutzeri
0.063
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I, pH 7.3, 25°C Pseudomonas stutzeri
0.064
-
 NAD+ mutant Q215L, pH 7.3, 25°C Pseudomonas stutzeri
0.065
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/D162N/V315A, pH 7.3, 25°C Pseudomonas stutzeri
0.066
-
 NAD+ mutant Q137R, pH 7.3, 25°C Pseudomonas stutzeri
0.066
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A, pH 7.3, 25°C Pseudomonas stutzeri
0.066
-
 NAD+ wild-type, pH 7.3, 25°C Pseudomonas stutzeri
0.07
-
 NAD+ mutant R275Q, pH 7.3, 25°C Pseudomonas stutzeri
0.071
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L, pH 7.3, 25°C Pseudomonas stutzeri
0.074
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C Pseudomonas stutzeri
0.075
-
 NAD+ mutant I150F, pH 7.3, 25°C Pseudomonas stutzeri
0.075
-
 phosphite mutant Q132R/Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C Pseudomonas stutzeri
0.075
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K, pH 7.3, 25°C Pseudomonas stutzeri
0.078
-
 phosphite mutant R275Q, pH 7.3, 25°C Pseudomonas stutzeri
0.092
-
 phosphite mutant Q137R/I150F/Q215L/R275Q/A319E, pH 7.3, 25°C Pseudomonas stutzeri
0.096
-
 phosphite mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A, pH 7.3, 25°C Pseudomonas stutzeri
0.099
-
 phosphite mutant I150F, pH 7.3, 25°C Pseudomonas stutzeri
0.105
-
 phosphite mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I, pH 7.3, 25°C Pseudomonas stutzeri
0.12
-
 phosphite mutant Q137R/I150F/Q215L/R275Q/L276Q, pH 7.3, 25°C Pseudomonas stutzeri
0.123
-
 phosphite mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K, pH 7.3, 25°C Pseudomonas stutzeri
0.135
-
 phosphite mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V, pH 7.3, 25°C Pseudomonas stutzeri
0.144
-
 phosphite mutant Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C Pseudomonas stutzeri
0.155
-
 phosphite mutant Q137R/I150F/Q215L/R275Q/D162N/V315A, pH 7.3, 25°C Pseudomonas stutzeri
0.156
-
 phosphite mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L, pH 7.3, 25°C Pseudomonas stutzeri
0.169
-
 phosphite mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R, pH 7.3, 25°C Pseudomonas stutzeri

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphite + H2O + NAD+
-
 Pseudomonas stutzeri phosphate + NADH + H+
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
39
-
 wild-type, melting temperature Pseudomonas stutzeri
59.3
-
 mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V, melting temperature Pseudomonas stutzeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8
-
 NAD+ mutant Q132R/Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C Pseudomonas stutzeri
2.85
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/D162N/V315A, pH 7.3, 25°C Pseudomonas stutzeri
2.9
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q, pH 7.3, 25°C Pseudomonas stutzeri
2.9
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A, pH 7.3, 25°C Pseudomonas stutzeri
3.13
-
 NAD+ mutant Q132R, pH 7.3, 25°C Pseudomonas stutzeri
3.2
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/A319E, pH 7.3, 25°C Pseudomonas stutzeri
3.2
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R, pH 7.3, 25°C Pseudomonas stutzeri
3.2
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V, pH 7.3, 25°C Pseudomonas stutzeri
3.5
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V, pH 7.3, 25°C Pseudomonas stutzeri
3.5
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K, pH 7.3, 25°C Pseudomonas stutzeri
3.5
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L, pH 7.3, 25°C Pseudomonas stutzeri
3.5
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I, pH 7.3, 25°C Pseudomonas stutzeri
3.6
-
 NAD+ mutant Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C Pseudomonas stutzeri
3.96
-
 NAD+ mutant Q132R, pH 7.3, 25°C Pseudomonas stutzeri
4.1
-
 NAD+ mutant R275Q, pH 7.3, 25°C Pseudomonas stutzeri
4.4
-
 NAD+ mutant I150F, pH 7.3, 25°C Pseudomonas stutzeri
4.4
-
 NAD+ wild-type, pH 7.3, 25°C Pseudomonas stutzeri
4.6
-
 NAD+ mutant Q215L, pH 7.3, 25°C Pseudomonas stutzeri
4.75
-
 NAD+ mutant Q137R, pH 7.3, 25°C Pseudomonas stutzeri