Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.21.3.1 extracted from

  • Roach, P.L.; Clifton, I.J.; Fulop, V.; Harlos, K.; Barton, G.J.; Hajdu, J.; Andersson, I.; Schofield, C.J.; Baldwin, J.E.
    Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes (1995), Nature, 375, 700-704.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
O2 required Aspergillus nidulans

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme complexed with manganese instead of iron in the active site, more stable Aspergillus nidulans
structure analysis Aspergillus nidulans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Aspergillus nidulans common step in the biosynthesis of penicillins, cephalosporins and cephamycins isopenicillin N + 2 H2O product has antibiotic activity ?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans P05326 accession number SwissProt
-

Reaction

Reaction Comment Organism Reaction ID
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O structure and mechanism Aspergillus nidulans
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O active site with conserved jelly-roll motif, cysteine residues are not directly involved in the coordination of the metal ion Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
Aspergillus nidulans isopenicillin N + 2 H2O
-
?
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 common step in the biosynthesis of penicillins, cephalosporins and cephamycins Aspergillus nidulans isopenicillin N + 2 H2O product has antibiotic activity ?

Synonyms

Synonyms Comment Organism
IPNS
-
Aspergillus nidulans