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Literature summary for 1.21.4.1 extracted from

  • Abeles, R.H.
    D-Proline reductase (Clostridium sticklandii) (1971), Methods Enzymol., 17, 317-321.
No PubMed abstract available
Search for more literature for 1.21.4.1

Activating Compound

Activating Compound Comment Organism Structure
pyruvate covalently bound Acetoanaerobium sticklandii

General Stability

General Stability Organism
stability is greatly enhanced by polyvalent ions such as SO42- or HPO42- Acetoanaerobium sticklandii

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine
-
 Acetoanaerobium sticklandii
iodoacetamide
-
 Acetoanaerobium sticklandii
iodoacetate
-
 Acetoanaerobium sticklandii
NaBH4 irreversible inactivation Acetoanaerobium sticklandii
phenylhydrazine
-
 Acetoanaerobium sticklandii

Organism

Organism UniProt Comment Textmining
Acetoanaerobium sticklandii
-
 strain ATCC12662 identical with strain DSM 519 T
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acetoanaerobium sticklandii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.71
-
-
 Acetoanaerobium sticklandii

Storage Stability

Storage Stability Organism
0-5°C purified enzyme stable for at least 1 month Acetoanaerobium sticklandii
0°C frozen in solution retains constant enzyme activity for at least a week Acetoanaerobium sticklandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-proline + dithiol (reduced)
-
 Acetoanaerobium sticklandii 5-aminopentanoic acid + oxidized dithiol
-
 ir

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.6
-
 maximum stability Acetoanaerobium sticklandii