Cloned (Comment) | Organism |
---|---|
cloning and sequencing of a new gene region, encoding a proprotein for the beta and alpha subunits of selenoprotein B: grdE, selenoprotein A: grdA and selenium-containing gamma subunit of selenoprotein B: grdB | Peptoclostridium acidaminophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
selenoprotein B, alpha,beta,gamma, 2 * 22000 + 2 * 25000 + 2 * 47000 | Peptoclostridium acidaminophilum |
25000 | - |
selenoprotein B, alpha,beta,gamma, 2 * 22000 + 2 * 25000 + 2 * 47000 | Peptoclostridium acidaminophilum |
47000 | - |
selenoprotein B, alpha,beta,gamma, 2 * 22000 + 2 * 25000 + 2 * 47000 | Peptoclostridium acidaminophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O | Peptoclostridium acidaminophilum | - |
glycine + phosphate + thioredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Peptoclostridium acidaminophilum | Q9R4G7 | DSM 5388T, growth on serine | - |
Purification (Comment) | Organism |
---|---|
of selenoprotein B of enzyme | Peptoclostridium acidaminophilum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | mechanism | Peptoclostridium acidaminophilum | |
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | cleavage mechanism for the pyruvoyl group dependent reductase starting from cysteine | Peptoclostridium acidaminophilum | |
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | The reaction is observed only in the direction of glycine reduction. The enzyme consists of three protein components A, B and C. Protein B contains selenocysteine and a pyruvoyl group, and is responsible for glycine binding and ammonia release. Protein A, which also contains selenocysteine, is reduced by thioredoxin, and is needed to convert the carboxymethyl group into a ketene equivalent, in turn used by protein C to produce acetyl phosphate. Only protein B distinguishes this enzyme from EC 1.21.4.3 (sarcosine reductase) and EC 1.21.4.4 (betaine reductase) | Peptoclostridium acidaminophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
352 | - |
substrate-specific selenoprotein B of enzyme | Peptoclostridium acidaminophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O | - |
Peptoclostridium acidaminophilum | glycine + phosphate + thioredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | selenoprotein B, alpha,beta,gamma, 2 * 22000 + 2 * 25000 + 2 * 47000 | Peptoclostridium acidaminophilum |