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Literature summary for 1.21.4.2 extracted from

  • Tanaka, H.; Stadtman, T.C.
    Selenium-dependent clostridial glycine reductase. Purification and characterization of the two membrane-associated protein components (1979), J. Biol. Chem., 254, 447-452.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
(NH4)2SO4
-
Acetoanaerobium sticklandii

Inhibitors

Inhibitors Comment Organism Structure
KBH4 inactivates protein B, very little effect on fraction C Acetoanaerobium sticklandii
LiCl
-
Acetoanaerobium sticklandii
additional information no effect: CaCl2, CoCl2, MnCl2 Acetoanaerobium sticklandii
NaCl
-
Acetoanaerobium sticklandii
NH2OH inhibits protein B and fraction C Acetoanaerobium sticklandii
NH4Cl
-
Acetoanaerobium sticklandii
Zn(COOH)2
-
Acetoanaerobium sticklandii
ZnCl2
-
Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl phosphate + NH3 + thioredoxin disulfide + H2O Acetoanaerobium sticklandii
-
glycine + phosphate + thioredoxin
-
?

Organism

Organism UniProt Comment Textmining
Acetoanaerobium sticklandii
-
-
-

Reaction

Reaction Comment Organism Reaction ID
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin mechanism Acetoanaerobium sticklandii
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin The reaction is observed only in the direction of glycine reduction. The enzyme consists of three protein components A, B and C. Protein B contains selenocysteine and a pyruvoyl group, and is responsible for glycine binding and ammonia release. Protein A, which also contains selenocysteine, is reduced by thioredoxin, and is needed to convert the carboxymethyl group into a ketene equivalent, in turn used by protein C to produce acetyl phosphate. Only protein B distinguishes this enzyme from EC 1.21.4.3 (sarcosine reductase) and EC 1.21.4.4 (betaine reductase) Acetoanaerobium sticklandii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5
-
protein B of enzyme Acetoanaerobium sticklandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl phosphate + NH3 + thioredoxin disulfide + H2O
-
Acetoanaerobium sticklandii glycine + phosphate + thioredoxin
-
?
acetyl phosphate + NH3 + thioredoxin disulfide + H2O reduction of glycine action is coupled with formation of ATP from ADP Acetoanaerobium sticklandii glycine + phosphate + thioredoxin
-
?