Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.21.98.1 extracted from

  • Cooper, L.E.; Fedoseyenko, D.; Abdelwahed, S.H.; Kim, S.H.; Dairi, T.; Begley, T.P.
    In vitro reconstitution of the radical S-adenosylmethionine enzyme MqnC involved in the biosynthesis of futalosine-derived menaquinon (2013), Biochemistry, 52, 4592-4594.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.21.98.1

Cloned(Commentary)

Cloned (Comment) Organism
recombinant plasmid encoding mqnC from Bacillus halodurans C-125 is introduced into Escherichia coli BL21(DE3)-T1R containing the Escherichia coli suf operon for coexpression of His6-MqnC with iron-sulfur cluster biogenesis proteins Halalkalibacterium halodurans

Metals/Ions

Metals/Ions Comment Organism Structure
[4Fe-4S] cluster 4Fe-4S cluster-dependent enzyme Halalkalibacterium halodurans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dehypoxanthine futalosine + S-adenosyl-L-methionine Halalkalibacterium halodurans
-
 cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
 ?
dehypoxanthine futalosine + S-adenosyl-L-methionine Halalkalibacterium halodurans C-125
-
 cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
 ?

Organism

Organism UniProt Comment Textmining
Halalkalibacterium halodurans Q9K864
-
-
Halalkalibacterium halodurans C-125 Q9K864
-
-

Purification (Commentary)

Purification (Comment) Organism
anaerobic purification by nickel affinity chromatography Halalkalibacterium halodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dehypoxanthine futalosine + S-adenosyl-L-methionine
-
 Halalkalibacterium halodurans cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
 ?
dehypoxanthine futalosine + S-adenosyl-L-methionine dehypoxanthine futalosine i.e. 3-[3-[(2R,3S,4R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl]benzoate Halalkalibacterium halodurans cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine cyclic dehypoxanthinyl futalosine i.e. (2R,3S,4R)-3,4,5-trihydroxy-4'-oxo-3',4,4',5-tetrahydro-2'H,3H-spiro[furan-2,1'-naphthalene]-6'-carboxylate ?
dehypoxanthine futalosine + S-adenosyl-L-methionine
-
 Halalkalibacterium halodurans C-125 cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
 ?
dehypoxanthine futalosine + S-adenosyl-L-methionine dehypoxanthine futalosine i.e. 3-[3-[(2R,3S,4R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl]benzoate Halalkalibacterium halodurans C-125 cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine cyclic dehypoxanthinyl futalosine i.e. (2R,3S,4R)-3,4,5-trihydroxy-4'-oxo-3',4,4',5-tetrahydro-2'H,3H-spiro[furan-2,1'-naphthalene]-6'-carboxylate ?

Synonyms

Synonyms Comment Organism
dehypoxanthinyl futalosine cyclase
-
 Halalkalibacterium halodurans
DHFL cyclase
-
 Halalkalibacterium halodurans
MqnC
-
 Halalkalibacterium halodurans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Halalkalibacterium halodurans

General Information

General Information Comment Organism
physiological function the enzyme is part of the futalosine pathway for menaquinone biosynthesis Halalkalibacterium halodurans