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Literature summary for 1.21.99.4 extracted from

  • Safran, M.; Leonard, J.L.
    Comparison of the physicochemical properties of type I and type II iodothyroine 5'-deiodinase (1991), J. Biol. Chem., 266, 3233-3238.
    View publication on PubMed

General Stability

General Stability Organism
activity mostly lost during purification procedures, catalytic activity lost in detergent solution Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
N-bromoacetyl-L-thyroxine i.e. BrAc-3,3',5-triiodothyronine, all isoenzymes Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane isoenzyme type I and type II are both integral membrane proteins Rattus sp. 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55400
-
type I, sucrose density gradient centrifugation and gel filtration Rattus sp.
198700
-
type II, sucrose density gradient centrifugation and gel filtration Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,3',5'-triiodothyronine + AH2 Rattus sp. isoenzyme type I 3,3'-diiodothyronine + iodide + A + H+
-
?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein no glycosylation of substrate binding subunits of both isoenzymes Rattus sp.

Purification (Commentary)

Purification (Comment) Organism
isoenzyme type I and type II Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
glial cell
-
Rattus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3',5'-triiodothyronine + AH2 isoenzyme type I Rattus sp. 3,3'-diiodothyronine + iodide + A + H+
-
?

Subunits

Subunits Comment Organism
More substrate binding subunit of multimeric isoenzyme type I has 27 kD and of multimeric isoenzyme type II 29 kD, SDS-PAGE Rattus sp.

Synonyms

Synonyms Comment Organism
iodothyronine 5'-deiodinase isoenzymes type I (D1) and type II (D2) Rattus sp.