Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes, in which selenocysteine residues in the core catalytic center is replaced by cysteine, in Saccharomyces cerevisiae | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant enzymes, in which selenocysteine residues in the core catalytic center is replaced by cysteine, shows a 10fold increase in Km-value for 3,3',5-triiodo-L-thyronine sulfate. Deletion of the endoplasmic reticulum (ER)-signal sequence and the membrane-spanning domain, amino acids 2-35, does not result in the production of a soluble type I-like enzyme. This mutant protein is inactive but is still membrane-bound | Rattus norvegicus |
N203Q | decrease in Km-value for 3,3',5-triiodo-L-thyronine sulfate | Rattus norvegicus |
N94Q | decrease in Km-value for 3,3',5-triiodo-L-thyronine sulfate | Rattus norvegicus |
N94Q/N203Q | increase in Km-value for 3,3',5-triiodo-L-thyronine sulfate | Rattus norvegicus |
S101A | Km-value for 3,3',5-triiodo-L-thyronine sulfate is nearly identical | Rattus norvegicus |
S101A/S176A | increase in Km-value for 3,3',5-triiodo-L-thyronine sulfate | Rattus norvegicus |
Y209F/Y217F | increase in Km-value for 3,3',5-triiodo-L-thyronine sulfate | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | mutant enzymes S101A/S176A and Y209F/Y217F show KM-values above 0.005 mM | Rattus norvegicus | |
0.0015 | - |
3,3',5-triiodo-L-thyronine sulfate | mutant enzyme G2A, duplicate determination give the values: 0.0015 mM and 0.0024 mM | Rattus norvegicus | |
0.0018 | - |
3,3',5-triiodo-L-thyronine sulfate | mutant enzyme N94Q | Rattus norvegicus | |
0.002 | - |
3,3',5-triiodo-L-thyronine sulfate | mutant enzyme S176A, duplicate determination give the values: 0.002 mM and 0.0024 mM | Rattus norvegicus | |
0.002 | - |
3,3',5-triiodo-L-thyronine sulfate | mutant enzyme S176A, duplicate determination give the values: 0.0024 mM and 0.0024 mM | Rattus norvegicus | |
0.002 | - |
3,3',5-triiodo-L-thyronine sulfate | mutant enzymes, in which selenocysteine residues in the core catalytic center is replaced by cysteine | Rattus norvegicus | |
0.0022 | - |
3,3',5-triiodo-L-thyronine sulfate | mutant enzyme S101A, duplicate determination give the values: 0.0022 mM and 3.2 mM | Rattus norvegicus | |
0.0024 | - |
3,3',5-triiodo-L-thyronine sulfate | mutant enzyme G2A, duplicate determination give the values: 0.0015 mM and 0.0024 mM | Rattus norvegicus | |
0.0028 | - |
3,3',5-triiodo-L-thyronine sulfate | native enzyme from rat liver microsomes | Rattus norvegicus | |
0.0029 | - |
3,3',5-triiodo-L-thyronine sulfate | mutant enzyme N94Q/N203Q | Rattus norvegicus | |
0.0032 | - |
3,3',5-triiodo-L-thyronine sulfate | mutant enzyme S101A, duplicate determination give the values: 0.0022 mM and 0.0032 mM | Rattus norvegicus | |
0.0045 | - |
3,3',5-triiodo-L-thyronine sulfate | mutant enzyme N203Q | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound to | Rattus norvegicus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Se | enzyme contains selenocysteine | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P24389 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | heterogeneity is not caused by N-linked glycosylation, but probably by a combination of O-linked glycosylation and phosphorylation | Rattus norvegicus |
phosphoprotein | heterogeneity is not caused by N-linked glycosylation, but probably by a combination of O-linked glycosylation and phosphorylation | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3',5-triiodo-L-thyronine sulfate + reduced electron acceptor | - |
Rattus norvegicus | diiodothyronine + iodide + electron acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
type I iodothyronine deiodinase | - |
Rattus norvegicus |