Literature summary for 1.23.1.3 extracted from

Min, T.; Kasahara, H.; Bedgar, D.L.; Youn, B.; Lawrence, P.K.; Gang, D.R.; Halls, S.C.; Park, H.; Hilsenbeck, J.L.; Davin, L.B.; Lewis, N.G.; Kang, C.
Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases and their relationship to isoflavone reductases (2003), J. Biol. Chem., 278, 50714-50723.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli B834-DE3 cells	Thuja plicata

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor diffusion method, using 20% (w/v) polyethylene glycol 8000, 0.1 M MES, pH 6.2, 0.1 M NaCl, and 0.1 M calcium acetate	Thuja plicata

Organism

Organism	UniProt	Comment	Textmining
Thuja plicata	-	-	-
Thuja plicata	Q9LD14	-	-

Purification (Commentary)

Purification (Comment)	Organism
POROS 20 HQ column chromatography and ADP-agarose column chromatography	Thuja plicata

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(-)-lariciresinol + NADPH + H+	-	Thuja plicata	(+)-secoisolariciresinol + NADP+	-	?
(-)-pinoresinol + NADPH + H+	-	Thuja plicata	(-)-lariciresinol + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
pinoresinol-lariciresinol reductase	-	Thuja plicata
PLR-Tp1	-	Thuja plicata
PLR-Tp2	-	Thuja plicata

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Thuja plicata

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Release 2023.1 (January 2023)