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Literature summary for 1.3.1.1 extracted from

  • Hidese, R.; Mihara, H.; Kurihara, T.; Esaki, N.
    Escherichia coli dihydropyrimidine dehydrogenase is a novel NAD-dependent heterotetramer essential for the production of 5,6-dihydrouracil (2011), J. Bacteriol., 193, 989-993.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 Uracil pH 6.0, 30°C Escherichia coli
0.087
-
 thymine pH 6.0, 30°C Escherichia coli
0.13
-
 Dihydrothymine pH 11.0, 30°C Escherichia coli
0.16
-
 5,6-dihydrouracil pH 11.0, 30°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44329
-
 2 * 44329, subunit PreT, plus 2 * 45069, subunit PreA Escherichia coli
45069
-
 2 * 44329, subunit PreT, plus 2 * 45069, subunit PreA Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P25889 subunit PreA
-
Escherichia coli P76440 subunit PreT
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dihydrouracil + NAD+
-
 Escherichia coli uracil + NADH + H+
-
 ?
5-fluorouracil + NADH + H+
-
 Escherichia coli 5-fluoro-5,6-dihydrouracil + NAD+
-
 r
dihydrothymine + NAD+
-
 Escherichia coli thymine + NADH + H+
-
 ?
additional information no substrate: orotate Escherichia coli ?
-
 ?
thymine + NADH + H+
-
 Escherichia coli dihydrothymine + NAD+
-
 r
uracil + NADH + H+
-
 Escherichia coli 5,6-dihydrouracil + NAD+
-
 r

Subunits

Subunits Comment Organism
tetramer 2 * 44329, subunit PreT, plus 2 * 45069, subunit PreA Escherichia coli

Synonyms

Synonyms Comment Organism
DPD
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.28
-
 Dihydrothymine pH 11.0, 30°C Escherichia coli
0.39
-
 thymine pH 6.0, 30°C Escherichia coli
0.64
-
 Uracil pH 6.0, 30°C Escherichia coli
0.65
-
 5,6-dihydrouracil pH 11.0, 30°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 reduction of uracil, assay at Escherichia coli
11
-
 oxidation of dihydrouracil, assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
iron-sulfur centre
-
 Escherichia coli
NAD+
-
 Escherichia coli
NADH specific for NADH Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8
-
 Dihydrothymine pH 11.0, 30°C Escherichia coli
4.5
-
 thymine pH 6.0, 30°C Escherichia coli
6.5
-
 5,6-dihydrouracil pH 11.0, 30°C Escherichia coli
17
-
 Uracil pH 6.0, 30°C Escherichia coli