Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4Cl | required for stability and activity. Assay in presence of 0.1 M NH4Cl | Porphyromonas gingivalis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Porphyromonas gingivalis |
General Stability | Organism |
---|---|
enzyme can be concentrated to 20 mg/ml without precipitation | Porphyromonas gingivalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Porphyromonas gingivalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0171 | - |
NADPH | pH 7.5, 25°C | Porphyromonas gingivalis | |
0.0478 | - |
crotonyl-[acyl-carrier protein] | pH 7.5, 25°C | Porphyromonas gingivalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33090 | - |
x * 33090, calculated | Porphyromonas gingivalis |
Organic Solvent | Comment | Organism |
---|---|---|
dimethyl sulfoxide | up to 10%, no loss of activity | Porphyromonas gingivalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | Q7MAW0 | - |
- |
Porphyromonas gingivalis W83 | Q7MAW0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Porphyromonas gingivalis |
Storage Stability | Organism |
---|---|
-20°C, 30% glycerol, no significant loss of activity for at least 1 month | Porphyromonas gingivalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-[acyl-carrier protein] + NADPH + H+ | - |
Porphyromonas gingivalis | butyryl-[acyl-carrier protein] + NADP+ | - |
? | |
crotonyl-[acyl-carrier protein] + NADPH + H+ | - |
Porphyromonas gingivalis W83 | butyryl-[acyl-carrier protein] + NADP+ | - |
? | |
additional information | enzyme possesses intrinsic NADPH oxidase activity in the absence of the crotonyl-CoA substrate | Porphyromonas gingivalis | ? | - |
? | |
additional information | enzyme possesses intrinsic NADPH oxidase activity in the absence of the crotonyl-CoA substrate | Porphyromonas gingivalis W83 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33090, calculated | Porphyromonas gingivalis |
Synonyms | Comment | Organism |
---|---|---|
enoyl-ACP-reductase II | - |
Porphyromonas gingivalis |
FabK | - |
Porphyromonas gingivalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Porphyromonas gingivalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Porphyromonas gingivalis | |
NADH | poor cofactor | Porphyromonas gingivalis | |
NADPH | inhibitory at high concentration | Porphyromonas gingivalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.109 | - |
NADPH | pH 7.5, 25°C | Porphyromonas gingivalis |