Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL1 Blue MRF cells | Aspergillus fumigatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 45000, SDS-PAGE | Aspergillus fumigatus |
46000 | - |
gel filtration | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | V5IVA1 | - |
- |
Aspergillus fumigatus B5233 | V5IVA1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chanoclavine-I aldehyde + NADH + H+ | in the presence of FgaFS only | Aspergillus fumigatus | dihydrochanoclavine-I aldehyde + NAD+ | - |
? | |
chanoclavine-I aldehyde + NADH + H+ | in the presence of FgaFS only | Aspergillus fumigatus B5233 | dihydrochanoclavine-I aldehyde + NAD+ | - |
? | |
additional information | the enzyme is more active in the presence of FMN and NADH than other combinations, e.g. FMN and NADPH, FAD and NADH or FAD and NADPH | Aspergillus fumigatus | ? | - |
? | |
additional information | the enzyme is more active in the presence of FMN and NADH than other combinations, e.g. FMN and NADPH, FAD and NADH or FAD and NADPH | Aspergillus fumigatus B5233 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
FgaOx3 | - |
Aspergillus fumigatus |
old yellow enzyme | - |
Aspergillus fumigatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Aspergillus fumigatus | |
NADH | - |
Aspergillus fumigatus |