Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
G23S | mutation increases the resistance of Staphylococcus aureus to triclosan by an order of magnitude | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hexachlorophene | - |
Staphylococcus aureus | |
triclosan | the introduction of a plasmid expressing the enzyme gene leads to triclosan resistance | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
crotonyl-N-acetylcysteamine | pH 7.5, 25°C | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q9RMI3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-N-acetylcysteamine + NADPH + H+ | - |
Staphylococcus aureus | butyryl-N-acetylcysteamine + NADP+ | - |
? | |
trans-2-octenoyl-N-acetylcysteamine + NADPH + H+ | - |
Staphylococcus aureus | octanoyl-N-acetylcysteamine + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FabI | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | exhibits specific and positive cooperative binding of NADPH. The cofactor concentration that yields 50% of the maximal rate is 65 microM | Staphylococcus aureus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.003 | - |
pH 7.5, 25°C | Staphylococcus aureus | triclosan |
General Information | Comment | Organism |
---|---|---|
physiological function | expression complements the Escherichia coli fabI temperature-sensitive mutant | Staphylococcus aureus |