Cloned (Comment) | Organism |
---|---|
overexpression | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | 100000 | chorismate mutase-prephenate dehydrogenase bifunctional enzyme, gel electrophoresis | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | Escherichia coli | biosynthesis of L-tyrosine | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chorismate mutase-prephenate dehydrogenase bifunctional enzyme | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
52 | 101 | - |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 0.1 M N-ethylmorpholine, pH 7.0, 21 mM citrate, 10% v/v glycerol, 1 mM EDTA, 1 mM DTT | Escherichia coli |
4°C, 0.1 M N-ethylmorpholine, pH 7.0, 21 mM citrate, 10% v/v glycerol, 1 mM EDTA, 1 mM DTT, up to 4 weeks | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | - |
Escherichia coli | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
prephenate + NAD+ | biosynthesis of L-tyrosine | Escherichia coli | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chorismate mutase-prephenate dehydrogenase bifunctional enzyme | complex with EC 5.4.99.5 | Escherichia coli |
tyrA | - |
Escherichia coli |