Application | Comment | Organism |
---|---|---|
additional information | Mycobacterium tuberculosis PDH is a monofunctional protein, does not possess any chorismate mutase activity unlike many other enteric bacteria | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
into tyrA-pET30a and expressed in Escherichia coli BL21(DE3) as His-rMtPDH | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | IC50: 0.01 mM | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
NAD+ | - |
Mycobacterium tuberculosis | |
0.41 | - |
prephenate | - |
Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
sequence analysis | Mycobacterium tuberculosis |
36420 | - |
MALDI-TOF studies of the recombinant His-rMtPDH | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-rMtPDH, to near homogeneity by Ni-NTA affinity chromatography, ca. 94% pure | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | - |
Mycobacterium tuberculosis | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
prephenate + NAD+ | - |
Mycobacterium tuberculosis H37Rv | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Mycobacterium tuberculosis |
tyrA | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
170.4 | - |
prephenate | - |
Mycobacterium tuberculosis | |
356.1 | - |
NAD+ | - |
Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
- |
Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
IC50: 0.01 mM | Mycobacterium tuberculosis | NaCl |