Cloned (Comment) | Organism |
---|---|
gene bedD encoding cis-benzene dihydrodiol dehydrogenase on the natural plasmid pHMT112, expressed in Escherichia coli | Pseudomonas putida |
sequenze similarity to glycerol dehydrogenase of Escherichia coli | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
4 * 39000, SDS-PAGE | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-1,2-dihydrobenzene-1,2-diol + NAD+ | Pseudomonas putida | - |
catechol + NADH | - |
? | |
cis-1,2-dihydrobenzene-1,2-diol + NAD+ | Pseudomonas putida ML2 | - |
catechol + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida ML2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
dye-affinity chromatography and NAD+ agarose chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol + NAD+ | - |
Pseudomonas putida | propen-1,2-diol + NADH | - |
? | |
1,2-propanediol + NAD+ | - |
Pseudomonas putida ML2 | propen-1,2-diol + NADH | - |
? | |
cis-1,2-cyclohexanediol + NAD+ | - |
Pseudomonas putida | cyclohex-1-ene-1,2-diol + NADH | - |
? | |
cis-1,2-cyclohexanediol + NAD+ | - |
Pseudomonas putida ML2 | cyclohex-1-ene-1,2-diol + NADH | - |
? | |
cis-1,2-dihydrobenzene-1,2-diol + NAD+ | - |
Pseudomonas putida | catechol + NADH | - |
? | |
cis-1,2-dihydrobenzene-1,2-diol + NAD+ | - |
Pseudomonas putida ML2 | catechol + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 39000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
cis-benzene dihydrodiol dehydrogenase | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida |