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Literature summary for 1.3.1.19 extracted from

  • Fong, K.P.Y.; Goh, C.B.H.; Tan, H.M.
    Characterization and expression of the plasmid-borne bedD gene from Pseudomonas putida ML2, which codes for a NAD+-dependent cis-benzene dihydrodiol dehydrogenase (1996), J. Bacteriol., 178, 5592-5601.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene bedD encoding cis-benzene dihydrodiol dehydrogenase on the natural plasmid pHMT112, expressed in Escherichia coli Pseudomonas putida
sequenze similarity to glycerol dehydrogenase of Escherichia coli Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
4 * 39000, SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas putida
-
catechol + NADH
-
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas putida ML2
-
catechol + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida ML2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
dye-affinity chromatography and NAD+ agarose chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propanediol + NAD+
-
Pseudomonas putida propen-1,2-diol + NADH
-
?
1,2-propanediol + NAD+
-
Pseudomonas putida ML2 propen-1,2-diol + NADH
-
?
cis-1,2-cyclohexanediol + NAD+
-
Pseudomonas putida cyclohex-1-ene-1,2-diol + NADH
-
?
cis-1,2-cyclohexanediol + NAD+
-
Pseudomonas putida ML2 cyclohex-1-ene-1,2-diol + NADH
-
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
Pseudomonas putida catechol + NADH
-
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
Pseudomonas putida ML2 catechol + NADH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 39000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
cis-benzene dihydrodiol dehydrogenase
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas putida