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Literature summary for 1.3.1.2 extracted from
- Properties and assay of dihydrouracil dehydrogenase of rat liver (1960), J. Biol. Chem., 235, 719-725.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
Uracil | - |
Rattus norvegicus |  |
| 0.017 | - |
NADPH | - |
Rattus norvegicus | |
| 0.6 | - |
Dihydrothymine | in presence of dihydrouracil | Rattus norvegicus | |
| 2 | - |
5,6-dihydrouracil | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Rattus norvegicus | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + NADP+ | Rattus norvegicus | - |
uracil + NADPH | - |
r | |
| uracil + NADPH | Rattus norvegicus | rate limiting step in catabolic breakdown of uracil to beta-alanine | 5,6-dihydrouracil + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, 20% loss of activity after 1 month | Rattus norvegicus |
| -20°C, 2 mg/ml protein, 10% loss of activity after 2 weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + NADP+ | - |
Rattus norvegicus | uracil + NADPH | - |
r | |
| uracil + NADPH | - |
Rattus norvegicus | 5,6-dihydrouracil + NADP+ | - |
r | |
| uracil + NADPH | rate limiting step in catabolic breakdown of uracil to beta-alanine | Rattus norvegicus | 5,6-dihydrouracil + NADP+ | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.6 | uracil reduction | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 7.9 | about 80% of activity maximum at pH 6.7 and 7.9, uracil reduction | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Rattus norvegicus | |
| NADPH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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