Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sulfide | 23.4 mol per mol of enzyme | Cupriavidus necator |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-aminopyridine ADP | - |
Cupriavidus necator | |
5,6-dihydrouracil | - |
Cupriavidus necator | |
ATP-ribose | dead-end inhibition | Cupriavidus necator | |
additional information | not: thio-NADPH, cyclo-NADPH, deamino-NADPH | Cupriavidus necator | |
NADP+ | competitive versus NADPH | Cupriavidus necator | |
NADPH | - |
Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0049 | - |
Uracil | - |
Cupriavidus necator | |
0.0068 | - |
NADPH | in presence of uracil | Cupriavidus necator |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron atoms are probably present in iron-sulfur centers | Cupriavidus necator | |
Iron | 24.5 mol iron per mol of enzyme | Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
4 * 52000, SDS-PAGE | Cupriavidus necator |
207000 | 210000 | gel fitration or gradient native PAGE | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil + NADPH | Cupriavidus necator | rate limiting step in catabolic breakdown of uracil to beta-alanine | 5,6-dihydrouracil + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cupriavidus necator |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,6-dihydrouracil + NADP+ = uracil + NADPH + H+ | abstraction of pro-S hydrogen of NADPH and thus B-side stereospecific class dehydrogenase | Cupriavidus necator |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
182.1 | - |
purified enzyme | Cupriavidus necator |
Storage Stability | Organism |
---|---|
-80°C, 35 mM potassium phosphate, pH 7.4, 1 mM dithioerythritol, more than 2 months without loss of activity, without dithioerythritol 50% loss of activity | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminouracil + NADPH | - |
Cupriavidus necator | ? | - |
? | |
5-fluorouracil + NADPH + H+ | - |
Cupriavidus necator | 5-fluoro-5,6-dihydrouracil + NADP+ | - |
? | |
dihydrothymine + NADP+ | - |
Cupriavidus necator | thymine + NADPH | - |
r | |
additional information | not: orotate, cytosine | Cupriavidus necator | ? | - |
? | |
uracil + NADPH | - |
Cupriavidus necator | 5,6-dihydrouracil + NADP+ | - |
? | |
uracil + NADPH | rate limiting step in catabolic breakdown of uracil to beta-alanine | Cupriavidus necator | 5,6-dihydrouracil + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 52000, SDS-PAGE | Cupriavidus necator |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | 4.1 mol FMN per mole of enzyme | Cupriavidus necator | |
NADP+ | - |
Cupriavidus necator | |
NADPH | - |
Cupriavidus necator |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki for ATP-ribose, 3-aminopyridine ADP, and 2,6-dihydrouracil | Cupriavidus necator | |
additional information | - |
additional information | inhibitory reactions between uracil, NADP+ and dihydrouracil | Cupriavidus necator | |
0.007 | - |
NADPH | - |
Cupriavidus necator | |
0.011 | - |
NADP+ | - |
Cupriavidus necator | |
0.15 | - |
dihydrouracil | - |
Cupriavidus necator |